Vector development for the expression of foreign proteins in the vaccine strain Brucella abortus S19
Vector development for the expression of foreign proteins in the vaccine strain Brucella abortus S19
复制标题
DOI：
10.1128/iai.66.8.3862-3866.1998
复制
发表时间：
1998-08-01
影响因子：
3.1
通讯作者：
Ugalde, RA
中科院分区：
文献类型：
--
作者：
Comerci, DJ;Pollevick, GD;Ugalde, RA
利用含有布鲁氏菌bcsp 31基因调控序列和信号肽的DNA片段，构建了在疫苗株S19中表达外源抗原的载体。将该片段克隆到广宿主范围质粒pBBR 4MCS中，得到质粒pBEV。以克氏锥虫的重复抗原为报告蛋白，重组融合蛋白在布鲁氏菌中稳定表达，并分泌到布鲁氏菌周质间隙，诱导了良好的抗克氏锥虫抗体反应。cruzi抗原重复抗原在布鲁氏菌中的表达既不改变其生长模式，也不产生毒性或致死作用，在实验感染过程中，这种策略用于活重组疫苗的产生和B的标记。流产S19疫苗。这是首次在布鲁氏菌的周质中表达重组蛋白。
A vector for the expression of foreign antigens in the vaccine strain Brucella abortus S19 was developed by using a DNA fragment containing the regulatory sequences and the signal peptide of the Brucella bcsp31 gene. This fragment was cloned in broad-host-range plasmid pBBR4MCS, resulting in plasmid pBEV. As a reporter protein, a repetitive antigen of Trypanosoma cruzi was used, The recombinant fusion protein is stably expressed and secreted into the Brucella periplasmic space, inducing a good antibody response against the T. cruzi antigen. The expression of the repetitive antigen in Brucella neither altered its growth pattern nor generated a toxic or lethal effect during experimental infection, The application of this strategy for the generation of live recombinant vaccines and the tagging of B. abortus S19 vaccine is discussed. This is the first time that a recombinant protein has been expressed in the periplasm of brucellae.