Transcriptomic Responses to Coaggregation between Streptococcus gordonii and Streptococcus oralis.
Transcriptomic Responses to Coaggregation between Streptococcus gordonii and Streptococcus oralis.
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DOI：
10.1128/aem.01558-21
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发表时间：
2021-10-28
影响因子：
4.4
通讯作者：
Jakubovics NS
中科院分区：
文献类型：
--
作者：
Choo SW;Mohammed WK;Mutha NVR;Rostami N;Ahmed H;Krasnogor N;Tan GYA;Jakubovics NS
口腔细菌之间的细胞粘附在牙菌斑等多微生物群落的形成中起着关键作用。口腔链球菌，如戈登链球菌和口腔链球菌是牙菌斑的重要早期定植菌，并与多种不同的口腔微生物结合，形成多物种团块或“共聚集体”。金曲霉通过调节基因表达积极响应共聚集。为了进一步了解这些反应，我们评估了gordonii和S. oral在25%的人类唾液中共聚集后的基因调控。混合形成共聚集体，30 min后提取RNA进行双转录组测序（RNA- seq）分析。在口腔链球菌中，共聚集调控了18个基因（6个上调，12个下调）。编码氨基酸和抗生素生物合成、核糖体和中央碳代谢等功能的基因显著下调。总共有28个基因在戈登链球菌中被差异调控（25个上调，3个下调）。许多与转运蛋白和双组分（NisK/SpaK）调控系统相关的基因在共聚集后被上调。我们对gordoni - s。与不同的先前发表的S. gordonii配对（S. gordonii-核梭杆菌和S. gordonii-细小细孔菌）的口腔菌表明，基因调控是特异性的每对配对，反应似乎并不保守。这种区分邻近细菌的能力可能对S. gordonii在复杂生物膜（如牙菌斑）的发育过程中适当适应很重要。牙菌斑是人类最常见的两种疾病——龋齿和牙周炎的罪魁祸首。控制牙菌斑的形成和防止从口腔健康到疾病的转变需要对微生物定植和生物膜发育有详细的了解。链球菌是牙菌斑最常见的宿主之一。这项研究确定了当口腔链球菌相互结合时受到调节的关键基因，就像它们在牙菌斑形成的早期阶段一样。我们表明，特定基因在两种不同的口腔链球菌形成混合物种聚集体后受到调节。哥氏链球菌对与口腔链球菌共聚集的特异性反应不同于与其他口腔细菌共聚集的特异性反应。靶向在种间相互作用中上调的关键基因可能是控制生物膜发育和维持口腔健康的有力途径。
Cell-cell adhesion between oral bacteria plays a key role in the development of polymicrobial communities such as dental plaque. Oral streptococci such as Streptococcus gordonii and Streptococcus oralis are important early colonizers of dental plaque and bind to a wide range of different oral microorganisms, forming multispecies clumps or “coaggregates.” S. gordonii actively responds to coaggregation by regulating gene expression. To further understand these responses, we assessed gene regulation in S. gordonii and S. oralis following coaggregation in 25% human saliva. Coaggregates were formed by mixing, and after 30 min, RNA was extracted for dual transcriptome sequencing (RNA-Seq) analysis. In S. oralis, 18 genes (6 upregulated and 12 downregulated) were regulated by coaggregation. Significantly downregulated genes encoded functions such as amino acid and antibiotic biosynthesis, ribosome, and central carbon metabolism. In total, 28 genes were differentially regulated in Streptococcus gordonii (25 upregulated and 3 downregulated). Many genes associated with transporters and a two-component (NisK/SpaK) regulatory system were upregulated following coaggregation. Our comparative analyses of S. gordonii-S. oralis with different previously published S. gordonii pairings (S. gordonii-Fusobacterium nucleatum and S. gordonii-Veillonella parvula) suggest that the gene regulation is specific to each pairing, and responses do not appear to be conserved. This ability to distinguish between neighboring bacteria may be important for S. gordonii to adapt appropriately during the development of complex biofilms such as dental plaque. IMPORTANCE Dental plaque is responsible for two of the most prevalent diseases in humans, dental caries and periodontitis. Controlling the formation of dental plaque and preventing the transition from oral health to disease requires a detailed understanding of microbial colonization and biofilm development. Streptococci are among the most common colonizers of dental plaque. This study identifies key genes that are regulated when oral streptococci bind to one another, as they do in the early stages of dental plaque formation. We show that specific genes are regulated in two different oral streptococci following the formation of mixed-species aggregates. The specific responses of S. gordonii to coaggregation with S. oralis are different from those to coaggregation with other oral bacteria. Targeting the key genes that are upregulated during interspecies interactions may be a powerful approach to control the development of biofilm and maintain oral health.