Genetic fate mapping reveals that the caudal ganglionic eminence produces a large and diverse population of superficial cortical interneurons.
Genetic fate mapping reveals that the caudal ganglionic eminence produces a large and diverse population of superficial cortical interneurons.
复制标题
DOI：
10.1523/jneurosci.4515-09.2010
复制
发表时间：
2010-02-03
期刊：
影响因子：
--
通讯作者：
Fishell G
中科院分区：
文献类型：
--
作者：
Miyoshi G;Hjerling-Leffler J;Karayannis T;Sousa VH;Butt SJ;Battiste J;Johnson JE;Machold RP;Fishell G
通过将诱导遗传命运定位策略与电生理分析相结合，我们系统地表征了起源于尾神经节隆起（CGE）的皮质gaba能中间神经元群体。有趣的是，与内侧神经节隆起（MGE）衍生的皮质中间神经元群体相比，这种胚胎结构的中间神经元的起始（E12.5）和峰值生成（E16.5）发生在发育的三天后。此外，与锥体细胞或mge衍生的皮层中间神经元不同，cge衍生的中间神经元不会以内向外的方式整合到皮层中，而是优先（75%）占据与出生日期无关的皮层浅层。与先前的估计相反，cge衍生的中间神经元在数量上（约占所有皮质中间神经元的30%）和多样性上（由至少9种不同的亚型组成）都要大得多。此外，我们还发现大量cge衍生的中间神经元，包括神经胶质亚型，表达糖蛋白Reelin。事实上，大多数cge来源的皮质中间神经元表达Reelin或血管活性肠多肽（VIP）。因此，结合先前的研究，我们现在已经确定了绝大多数皮层中间神经元亚型的空间和时间起源。
By combining an inducible genetic fate mapping strategy with electrophysiological analysis, we have systematically characterized the populations of cortical GABAergic interneurons that originate from the caudal ganglionic eminence (CGE). Interestingly, in comparison to medial ganglionic eminence (MGE)-derived cortical interneuron populations, the initiation (E12.5) and peak production (E16.5) of interneurons from this embryonic structure occurs three days later in development. Moreover, unlike either pyramidal cells or MGE-derived cortical interneurons, CGE-derived interneurons do not integrate into the cortex in an inside-out manner but preferentially (75%) occupy superficial cortical layers independent of birthdate. In contrast to previous estimates, CGE-derived interneurons are both considerably greater in number (around 30% of all cortical interneurons) and diversity (comprised by at least nine distinct subtypes). Furthermore, we have found that a large proportion of CGE-derived interneurons, including the neurogliaform subtype, express the glycoprotein Reelin. In fact, most CGE-derived cortical interneurons express either Reelin or vasoactive intestinal polypeptide (VIP). Thus, in conjunction with previous studies, we have now determined the spatial and temporal origins of the vast majority of cortical interneuron subtypes.