Structure and intracellular localization of mouse ADP-ribosylation factors type 1 to type 6 (ARF1-ARF6).
Structure and intracellular localization of mouse ADP-ribosylation factors type 1 to type 6 (ARF1-ARF6).
复制标题
小鼠 ADP-核糖基化因子 1 型至 6 型 (ARF1-ARF6) 的结构和细胞内定位。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
Kazuhisa Nakayama
中科院分区：
文献类型：
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作者：
Masahiro Hosaka;Kyoko Toda;H. Takatsu;Seiji Torii;Kazuo Murakami;Kazuhisa Nakayama
ADP-核糖基化因子（ARF）是一个小的GTP结合蛋白家族，被认为参与了包被转运囊泡的形成。尽管迄今为止已经在哺乳动物中报道了六个ARF序列，但尚不清楚单个生物体中存在多少ARF成员。在这项研究中，我们提供了第一个直接的证据，通过cDNA克隆的所有六个ARF成员在小鼠中的存在。这些蛋白质在哺乳动物物种中高度保守，并且北方印迹分析显示所有成员的mRNA普遍表达。用表位标记的ARF转染细胞显示，ARF 1-3显示核周高尔基定位，而ARF 4-6似乎广泛分散在整个细胞质中。这些结果表明，虽然所有的ARF蛋白在细胞功能中发挥基本的和关键的作用，他们参与不同的囊泡运输过程。
ADP-ribosylation factors (ARFs) are a family of small GTP-binding proteins that are proposed to be involved in the formation of coated transport vesicles. Although six ARF sequences have been reported in mammals to date, it has been unclear how many ARF members are present in a single organism. In this study, we provide the first direct evidence by cDNA cloning for the presence of all six ARF members in mouse. These proteins are highly conserved across mammalian species and Northern blot analysis revealed that mRNAs for all the members were expressed ubiquitously. Transfection of cells with epitope-tagged ARFs revealed that ARFs 1-3 displayed a perinuclear Golgi localization, while ARFs 4-6 appeared to be widely dispersed throughout the cytoplasm. These results suggest that although all the ARF proteins play fundamental and critical roles in cellular function, they are involved in different vesicular transport processes.