Oxidation of aromatic compounds in organic solvents with laccase from Trametes versicolor
Oxidation of aromatic compounds in organic solvents with laccase from Trametes versicolor
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栓菌漆酶在有机溶剂中氧化芳香族化合物
DOI：
10.1007/bf00252530
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发表时间：
1989
影响因子：
5
通讯作者：
A. Hüttermann
中科院分区：
文献类型：
--
作者：
O. Milstein;B. Nicklas;A. Hüttermann
摘要从Trametes versicolor纯化的漆酶在用水预饱和的疏水性溶剂和亲水性有机溶剂中氧化2,6-二甲氧基苯酚(2,6-DMP)和丁香醛连氮，前提是添加足够量的水。在通过凝胶珠过滤酶的过程中，通过将酶包埋在 Sepharose CL-6B 中来固定酶，从而提高了有机溶剂中漆酶测试的性能。凝胶-酶缔合已被证明在水预饱和溶剂中是稳定的。固定化漆酶在含水7%的有机溶剂中的效率是柠檬酸钾缓冲液中的10%~20%。有机溶剂中的固定化漆酶表现出良好的稳定性和对高温的高耐受性。
SummaryLaccase purified from Trametes versicolor oxidizes 2,6-dimethoxyphenol (2,6-DMP) and syringaldazine in hydrophobic solvents presaturated with water, and in hydrophilic organic solvents provided that a sufficient amount of water is added. Ease of performance of the laccase test in organic solvents is improved after immobilization of the enzyme by entrapping in Sepharose CL-6B during enzyme filtration through the gel beads. The gel-enzyme association has been shown to be stable in water-presaturated solvents. Efficiency of the immobilized laccase in organic solvents containing 7% water was 10%–20% of that in potassium-citrate buffer. Immobilized laccase in organic solvents showed good stability and high tolerance to elevated temperatures.