KNOTTED1 mRNA undergoes long-distance transport and interacts with movement protein binding protein 2C in pear (Pyrus betulaefolia)
KNOTTED1 mRNA undergoes long-distance transport and interacts with movement protein binding protein 2C in pear (Pyrus betulaefolia)
复制标题
DOI：
10.1007/s11240-014-0685-z
复制
发表时间：
2015-04-01
影响因子：
3
通讯作者：
Li, Tianzhong
中科院分区：
文献类型：
--
作者：
Duan, Xuwei;Zhang, Wenna;Li, Tianzhong
KNOTTED 1（KN 1）-like homologous box是一种同源结构域转录因子，在植物中通过胞间连丝进行细胞间运输，并通过韧皮部进行长距离运输。本研究以杜梨（Pyrus betulaefolia，PbMPB 2C）为砧木，以鸭梨（P. bretschneideri，Scion）为接穗，研究了KN 1在两种果树中的远距离运输及其受病毒运动蛋白结合蛋白PbMPB 2C的调控。结果表明，PbKN 1 mRNA存在于韧皮部的两个运输方向通过嫁接连接的组培嫁接植物和果园树苗。为了研究PbKN 1和PbMPB 2C之间的关系，我们进行了相互作用试验，以证明它们的物理相互作用。RNA-蛋白质下拉实验表明，PbKN 1 mRNA与PbMPB 2C蛋白在体外相互作用，但不与PbKN 1，PbKN 1抑制PbMPB 2C与PbKN 1 mRNA的结合。此外，使用p35 S：GFP-PbKN 1转基因烟草与野生型植物嫁接，我们发现PbKN 1的移动通过NtMPB 2C的沉默而增加。基于上述发现，我们首先证明了PbKN 1 mRNA的双向运输的证据，并提出PbMPB 2C可以作为韧皮部介导的PbKN 1 mRNA的长距离运输的负调节剂。
KNOTTED1 (KN1)-like homologous box is a homeodomain transcription factor that is transported intercellularly through plasmodesmata and over long distances via phloem transport in plants. In this study, we investigated KN1 long-distance transport and its regulation by the viral movement protein binding protein of 'Du Li' (Pyrus betulaefolia, PbMPB2C) in two species of fruit trees, 'Du Li' (rootstock) and 'Ya Li' (P. bretschneideri, scion), using grafting experiments. The results showed that PbKN1 mRNA was present in the phloem in both transport directions across the graft junctions of tissue-cultured grafted plants and orchard saplings. To investigate the relationship between PbKN1 and PbMPB2C, we conducted interaction assays to demonstrate their physical interaction. An RNA-protein pull-down assay revealed that PbKN1 mRNA interacted with PbMPB2C protein in vitro but not with PbKN1 and that PbKN1 repressed the binding of PbMPB2C to PbKN1 mRNA. Additionally, using p35S:GFP-PbKN1 transgenic tobacco grafted with wild-type plants, we found that the movement of PbKN1 was increased by the silencing of NtMPB2C. Based on the above findings, we first demonstrate evidence of the bidirectional transport of PbKN1 mRNA and propose that PbMPB2C could act as a negative regulator of the phloem-mediated long-distance transport of PbKN1 mRNA.