Dual hairpin-like molecular beacon based on coralyne-adenosine interaction for sensing melamine in dairy products
Dual hairpin-like molecular beacon based on coralyne-adenosine interaction for sensing melamine in dairy products
复制标题
基于珊瑚腺苷相互作用的双发夹状分子信标用于检测乳制品中的三聚氰胺
DOI：
10.1016/j.talanta.2014.05.046
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发表时间：
2014
期刊：
影响因子：
6.1
通讯作者：
Zhang Xiaojun
中科院分区：
文献类型：
--
作者：
Wang Guangfeng;Zhu Yanhong;Chen Ling;Zhang Xiaojun
基于三聚氰胺（MA）与胸腺嘧啶（Thymine）的偶联，提出了一种新型的双发夹状分子信标（MB），用于选择性和灵敏地检测MA。在该方案中，Coralyne和腺苷（A）之间的配位导致双发夹状MB，并且荧光团-猝灭剂对非常接近，导致荧光猝灭。在MA的作用下，它通过三重氢键与双发夹样MB环部分的胸腺嘧啶偶联，引发双发夹样MB的解离。荧光团与猝灭剂的空间分离诱导荧光发射的增强。在优化条件下，该传感器对MA的线性范围为8×10−9 ~ 1.6×10− 5 M（R2=0.9969），检测限为5 nM，比美国FDA和美国食品安全限量低约4000倍。对真实的牛奶样品进行了检测，结果令人满意。
This study presents a novel dual hairpin-like molecular beacon (MB) for the selective and sensitive detection of melamine (MA) based on the conjugation of MA and thymine. In this protocol, the coordination between coralyne and adenosine (A) leaded a dual hairpin-like MB and the fluorophore–quencher pair is close proximity resulting in the fluorescence quenching. With the addition of MA, it conjugated with thymine in the loop part of dual hairpin-like MB by triple H-bonds, triggering the dissociation of the dual hairpin-like MB. The resulting spatial separation of the fluorophore from quencher induced the enhancement in fluorescence emission. Under the optimized conditions, the sensor exhibited a wide linear range of 8×10−9–1.6×10−5M (R2=0.9969) towards MA, with a low detection limit of 5 nM, approximately 4000 times lower than the Drug Administration and the US Food estimated MA safety limit. The real milk samples were also investigated with a satisfying result.