Three-dimensional imaging of fertilization and early development.
Three-dimensional imaging of fertilization and early development.
复制标题
受精和早期发育的三维成像。
DOI：
10.1002/jemt.1060170403
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Schatten,G
中科院分区：
文献类型：
--
作者：
Holy,J;Simerly,C;Paddock,S;Schatten,G
生物显微镜领域最近取得了重大的技术进步，例如场发射低电压扫描电子显微镜和激光扫描共聚焦光学显微镜的发展。此外，显微镜数据的计算机处理正在彻底改变形态信息的成像方式。在本文中，我们说明了这种新技术可以用来检查受精和早期发育的三维事件的方法。不同类型的标本制备方案，使用棘皮动物和哺乳动物配子和胚胎，评估其准确保存这些标本的三维组织的能力，以通过低压扫描电子显微镜和激光扫描共聚焦光学显微镜进行成像。
The field of biological microscopy has recently enjoyed major technical advances, exemplified by the development of field‐emission low‐voltage scanning electron microscopes and laser scanning confocal light microscopes. In addition, computer processing of microscopical data is revolutionizing the way morphological information is imaged. In this paper, we illustrate methods by which this new technology can be used to examine events in fertilization and early development in three dimensions. Different types of specimen preparation protocols, using both echinoderm and mammalian gametes and embryos, are evaluated for their ability to preserve accurately the threedimensional organization of these specimens for imaging by both low‐voltage scanning electron microscopy and laser scanning confocal light microscopy.