Environment-Mediated Accumulation of Diacyl Lipoproteins over Their Triacyl Counterparts in Staphylococcus aureus
Environment-Mediated Accumulation of Diacyl Lipoproteins over Their Triacyl Counterparts in Staphylococcus aureus
复制标题
DOI：
10.1128/jb.00314-12
复制
发表时间：
2012-07-01
影响因子：
3.2
通讯作者：
Lee, Bok Luel
中科院分区：
文献类型：
--
作者：
Kurokawa, Kenji;Kim, Min-Su;Lee, Bok Luel
细菌脂蛋白被认为只存在于一种特定的脂质修饰结构中，如二酰基形式或三酰基形式。以金黄色葡萄球菌为例，最近广泛的基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱分析显示，金黄色葡萄球菌脂蛋白以α -氨基酰化三酰基形式存在。在这里，我们发现了在金黄色葡萄球菌中诱导缺乏α -氨基酰化的二酰基脂蛋白积累的条件。二酰基脂蛋白的积累需要多种条件，包括酸性pH值和后对数生长期。高温和高盐浓度加速了二酰基脂蛋白形式的积累。在pH值为6的金黄色葡萄球菌MW2细胞生长后的对数生长期，SitC脂蛋白几乎全部在其二酰基结构中而不是在其三酰基结构中被发现。这是首次报道表明环境介导脂质修饰的细菌脂蛋白结构改变。
Bacterial lipoproteins are believed to exist in only one specific lipid-modified structure, such as the diacyl form or the triacyl form, in each bacterium. In the case of Staphylococcus aureus, recent extensive matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry analysis revealed that S. aureus lipoproteins exist in the alpha-aminoacylated triacyl form. Here, we discovered conditions that induce the accumulation of diacyl lipoproteins that lack alpha-aminoacylation in S. aureus. The accumulation of diacyl lipoproteins required a combination of conditions, including acidic pH and a post-logarithmic-growth phase. High temperatures and high salt concentrations additively accelerated the accumulation of the diacyl lipoprotein form. Following a post-logarithmic-growth phase where S. aureus MW2 cells were grown at pH 6, SitC lipoprotein was found almost exclusively in its diacyl structure rather than in its triacyl structure. This is the first report showing that the environment mediates lipid-modified structural alterations of bacterial lipoproteins.