The septin CDCrel-1 binds syntaxin and inhibits exocytosis
The septin CDCrel-1 binds syntaxin and inhibits exocytosis
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DOI：
10.1038/8100
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发表时间：
1999-05-01
影响因子：
25
通讯作者：
Trimble, WS
中科院分区：
文献类型：
--
作者：
Beites, CL;Xie, H;Trimble, WS
septin是多种生物完成细胞质分裂所需的gtp酶，但其在细胞质分裂或其他细胞过程中的作用尚不清楚。在这里，我们描述了一种新发现的septin CDCrel-1的研究，它主要在神经系统中表达。该蛋白与膜组分相关，并且有相当一部分蛋白与突触囊泡共沉淀。在洗涤剂提取物中，CDCrel-1和另一种septin Nedd5通过SNARE相互作用域直接与syntaxin结合，从而与SNARE蛋白syntaxin发生免疫沉淀。转染野生型CDCrel-1抑制HIT-T15细胞的分泌，而GTPase显性阴性突变体则增强分泌。这些数据表明septin可能通过与syntaxin的相互作用来调节囊泡动力学。
Septins are GTPases required for the completion of cytokinesis in diverse organisms, yet their roles in cytokinesis or other cellular processes remain unknown. Here we describe studies of a newly identified septin, CDCrel-1, which is predominantly expressed in the nervous system. This protein was associated with membrane fractions, and a significant fraction of the protein copurified and coprecipitated with synaptic vesicles. In detergent extracts, CDCrel-1 and another septin, Nedd5, immunoprecipitated with the SNARE protein syntaxin by directly binding to syntaxin via the SNARE interaction domain. Transfection of HIT-T15 cells with wild-type CDCrel-1 inhibited secretion, whereas GTPase dominant-negative mutants enhanced secretion. These data suggest that septins may regulate vesicle dynamics through interactions with syntaxin.