Arp2/3-branched actin regulates microtubule acetylation levels and affects mitochondrial distribution
Arp2/3-branched actin regulates microtubule acetylation levels and affects mitochondrial distribution
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Arp2/3 分支肌动蛋白调节微管乙酰化水平并影响线粒体分布
DOI：
10.1242/jcs.226506
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发表时间：
2019-03-01
影响因子：
4
通讯作者：
Wu，Congying
中科院分区：
文献类型：
--
作者：
Shi，Peng;Wang，Yuan;Wu，Congying
摘要肌动蛋白和微管细胞骨架调节细胞形态，参与细胞器的运输和功能，以响应不同的环境线索。这两种细胞骨架之间精确的时空协调对于细胞的生存和运动至关重要。在这里，我们报告了一种新的肌动蛋白和微管之间的串扰，其中分支的肌动蛋白通过影响微管蛋白乙酰化水平来维持微管的组织，动力学和稳定性。我们观察到，乙酰化微管蛋白显着减少扰动后的Arp 2/3分支的肌动蛋白。我们随后发现HDAC 6通过改变其与微管蛋白和Arp 2/3-稳定剂coronin的相互作用参与了这一过程。我们进一步确定，动态平衡的分支肌动蛋白控制线粒体分布通过这种微管乙酰化依赖的机制。我们的研究结果揭示了细胞骨架网络的整体观点，突出了翻译后修饰作为细胞骨架相互调节的另一种可能形式，除了通过结构连接或上游信号通路建立的串扰。总结：我们报告的翻译后修饰介导的串扰Arp 2/3分支肌动蛋白和微管，调节线粒体分布。
ABSTRACT Actin and microtubule cytoskeletons regulate cell morphology, participate in organelle trafficking and function in response to diverse environmental cues. Precise spatial-temporal coordination between these two cytoskeletons is essential for cells to live and move. Here, we report a novel crosstalk between actin and microtubules, in which the branched actin maintains microtubule organization, dynamics and stability by affecting tubulin acetylation levels. We observed that acetylated tubulin significantly decreases upon perturbation of the Arp2/3-branched actin. We subsequently discover that HDAC6 participates in this process by altering its interaction with tubulin and the Arp2/3-stabilizer cortactin. We further identify that the homeostasis of branched actin controls mitochondrial distribution via this microtubule acetylation-dependent mechanism. Our findings shed new light on the integral view of cytoskeletal networks, highlighting post-translational modification as another possible form of cytoskeletal inter-regulation, aside from the established crosstalks through structural connection or upstream signaling pathways. Summary: We report a post-translational modification-mediated crosstalk between Arp2/3-branched actin and microtubules that regulates mitochondrial distribution.