Heparin inhibits dengue-2 virus infection of five human liver cell lines
Heparin inhibits dengue-2 virus infection of five human liver cell lines
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DOI：
10.1016/s0166-3542(02)00095-5
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发表时间：
2002-10-01
影响因子：
7.6
通讯作者：
Liu, HS
中科院分区：
文献类型：
--
作者：
Lin, YL;Leib, HY;Liu, HS
肝脏是登革病毒感染的主要靶器官，在登革出血热的免疫发病机制中起重要作用。以前，我们报道了五个人肝细胞系（HuH-7，HA 22 T，Hep 3B，PLC和Chang liver）具有不同程度的分化和致瘤性，对登革病毒感染表现出不同的易感性。在这里，我们证明，肝素，硫酸乙酰肝素（HS）的类似物，可以竞争与HS在细胞膜上的病毒结合，随后抑制复制登革-2和乙型脑炎病毒在肝癌和BHK-21细胞，分别。说明病毒与HS的结合是其入侵的重要过程。此外，肝素的抑制作用与细胞中病毒的感染性相关。总而言之，我们的结果表明HS是登革热和日本脑炎病毒复制的重要宿主成分，肝素可以有效地阻断这种复制。(C)2002 Elsevier Science B. V.保留所有权利。
Liver is suggested to be the major target of dengue virus infection and plays an important role in the immunopathogenesis of dengue hemorrhagic fever. Previously, we reported that five human liver cell lines (HuH-7, HA22T, Hep3B, PLC, and Chang liver) with various degrees of differentiation and tumorigenicity showed different susceptibility for dengue virus infection. Here, we demonstrate that heparin, an analogue of heparan sulfate (HS), can compete with HS on cell membrane for virus binding and subsequently inhibits the replication of dengue-2 and Japanese encephalitis viruses in hepatoma and BHK-21 cells, respectively. It indicates that the binding of these viruses with HS is an important process for their invasion. Moreover, the inhibitory effect of heparin correlates with the infectivity of the virus in the cells. All together, our results suggest that HS is an important host component for dengue and Japanese encephalitis virus replication, which can be effectively blocked by heparin. (C) 2002 Elsevier Science B.V. All rights reserved.