Molecular characterization of Haynaldia villosa chromatin in wheat lines carrying resistance to wheat curl mite colonization
Molecular characterization of Haynaldia villosa chromatin in wheat lines carrying resistance to wheat curl mite colonization
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DOI：
10.1007/bf00224062
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发表时间：
1996-10
影响因子：
5.4
通讯作者：
Q. Chen;R. Conner;A. Laroche
中科院分区：
文献类型：
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作者：
Q. Chen;R. Conner;A. Laroche
小麦-簇毛麦（Haynaldia villosa（L.）Schur，杂种系被测试为抗小麦卷螨（小麦条斑花叶病毒的载体）定殖的潜在来源。发现两个品系，Add 6V-1和Sub 6V-1是抗螨的。荧光原位杂交技术，利用总基因组DNA，从H。在未标记的小麦DNA存在下，证实了Add 6V-1是一个二体小麦-H。绒毛染色体附加系亚6V-1是一个同源的小麦-H。绒毛染色体易位系而不是置换。Sub 6V-1中的易位发生在小麦染色体和H.通过罗伯逊融合的错误分裂的着丝粒。只有H.根据基因组原位杂交信号和聚合酶链反应获得的特异性DNA片段，初步推断绒茧蜂参与了抗螨性的遗传控制。
Wheat-Haynaldia villosa(L.) Schur, hybrid lines were tested as potential sources of resistance to colonization by the wheat curl mite, the vector of wheat streak mosaic virus. Two lines, Add 6V-1 and Sub 6V-1, were found to be mite-resistant. Fluorescence in situ hybridization using total genomic DNA, fromH. villosain the presence of unlabelled wheat DNA, confirmed that Add 6V-1 is a disomic wheat-H. villosachromosome addition line. Sub 6V-1 turned out to be a homoeologous wheat-H. villosachromosome translocation line rather than a substitution. The translocation in Sub 6V-1 occurred between a wheat chromosome and a chromosome fromH. villosathrough Robertsonian fusion of misdivided centromeres. Only the short arm of the group 6 chromosome ofH. villosawas involved in the genetic control of mite resistance, a conclusion based on the genomic in situ hybridization signal and specific DNA fragments obtained by polymerase chain reaction.