Micro-structural geometry of thin films intended for the inner lumen of nerve conduits affects nerve repair
Micro-structural geometry of thin films intended for the inner lumen of nerve conduits affects nerve repair
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DOI：
10.1007/s10856-013-4922-5
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发表时间：
2013-07-01
影响因子：
3.7
通讯作者：
Downes, S.
中科院分区：
文献类型：
--
作者：
Mobasseri, S. A.;Terenghi, G.;Downes, S.
周围神经的损伤可引起严重的运动或感觉损伤。在严重的情况下，从身体的另一部分牺牲神经来修复受损的神经（自体移植）。生物可降解聚合物导管的发展可能为自体移植物提供另一种选择。本研究研究了超薄聚（ε -己内酯）/聚乳酸共混溶剂铸膜的光滑、凹陷和沟槽结构的表面形貌和力学性能。我们研究了凹槽形状对细胞形态和排列的影响。采用光刻和干/湿蚀刻技术，开发出具有精确几何形状（V形、斜壁和方形）的凹槽的图案硅衬底。利用神经细胞系NG108-15进行体外实验，证实该聚合物支架具有良好的细胞附着和增殖能力。成像技术表明，根据凹槽的形状，有不同的细胞反应和形态。研究表明，几何形状，特别是斜坡的角度和凹槽之间的空间，会影响细胞的反应。此外，生物力学研究表明，图案膜具有优异的力学性能，比天然神经更强。导管是通过在芯轴周围滚动薄膜并使用热焊接技术将边缘连接起来制成的。有希望的生物力学和体外实验结果表明，神经细胞的反应受到纵向沟槽形状的影响，尤其是沟槽壁斜率的角度。
Damage to peripheral nerves can cause significant motor or sensory injuries. In serious cases, a nerve is sacrificed from another part of the body to repair a damaged nerve (autograft). The development of biodegradable polymer conduits may offer an alternative to autografts. This study investigated the surface topography and mechanical properties of smooth, pitted and grooved structures of ultra-thin poly (epsilon-caprolactone)/poly lactic acid blended, solvent-cast films. We have investigated the effect of the groove shape on cell morphology and alignment. Photolithography and dry/wet etching was used to develop patterned silicon substrates with grooves with accurate geometries (V shaped, sloped walls and square shaped). Using a neural cell line (NG108-15), in vitro experiments confirmed good cell attachment and proliferation on all the polymer scaffolds. Imaging techniques demonstrated that there was different cellular responses and morphology according to the shape of the groove. Studies showed that the geometry, particularly the angle of the slope and the space between grooves, affected cellular responses. In addition, biomechanical studies showed that the patterned films had excellent mechanical properties and were stronger than the natural nerve. The conduit tubes were made by rolling the films around a mandrel and using a thermal welding technique to join the edges. The promising biomechanical and in vitro results demonstrate that nerve cell responses are affected by the shape of longitudinal grooves, and particularly by the angle of the slope of the groove walls.