The late promoter of the human cytomegalovirus viral DNA polymerase processivity factor has an impact on delaye early and late viral gene products but not on viral DNA synthesis
The late promoter of the human cytomegalovirus viral DNA polymerase processivity factor has an impact on delaye early and late viral gene products but not on viral DNA synthesis
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DOI：
10.1128/jvi.00089-07
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发表时间：
2007-06-01
影响因子：
5.4
通讯作者：
Tsurumi, Tatsuya
中科院分区：
文献类型：
--
作者：
Isomura, Hiroki;Stinski, Mark F.;Tsurumi, Tatsuya
人巨细胞病毒DNA聚合酶持续性因子基因（UL44）的转录起始于三个不同的起始位点，在生产性感染过程中受到不同的调控。其中两个起始位点，即远端位点和近端位点，在感染早期有活性，而中间起始位点仅在感染后期有活性（F.利奇和E.S.莫卡尔斯基，《病毒学杂志》63：1783 - 1791，1989年）。与野生型相比，远端或中间TATA元件突变的重组病毒的UL44基因表达较低。从远端或中间起始位点起始的转录本促进了病毒晚期基因的表达。病毒DNA合成水平受到远端TATA元件突变的影响。相反，中间TATA元件的突变不影响病毒DNA合成水平，但确实显著影响病毒晚期基因的表达水平。在低感染复数和高感染复数下，远端或中间TATA元件突变的重组病毒比野生型生长更慢。来自晚期中间病毒启动子的UL44基因表达降低与晚期病毒蛋白表达减少和病毒生长减缓相关。
Transcription of the DNA polymerase processivity factor gene (UL44) of human cytomegalovirus initiates at three distinct start sites, which are differentially regulated during productive infection. Two of these start sites, the distal and proximal sites, are active at early times, and. the middle start site is active at only late times after infection (F. Leach and E. S. Mocarski, J. Virol. 63:1783-1791, 1989). Compared to the wild type, UL44 gene expression was lower for recombinant viruses with the distal or the middle TATA element mutated. The transcripts initiating from the distal or middle start site facilitated late viral gene expression. The level of viral DNA synthesis was affected by mutation of the distal TATA element. In contrast, mutation of the middle TATA element did not affect the level of viral DNA synthesis, but it did affect significantly the level of late viral gene expression. Recombinant viruses with the distal or middle TATA element mutated grew more slowly than the wild type at both low and high multiplicities of infection. Reduced expression of the UL44 gene from the late middle viral promoter correlated with decreased late viral protein expression and decreased viral growth.