A transcriptional Regulator Gar Regulates the Growth and Virulence of Xanthomonas oryzae pv. oryzae
A transcriptional Regulator Gar Regulates the Growth and Virulence of Xanthomonas oryzae pv. oryzae
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DOI：
10.1007/s00284-023-03396-9
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发表时间：
2023-09-01
影响因子：
2.6
通讯作者：
Ruan，Lifang
中科院分区：
文献类型：
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作者：
Wang，Huihui;Chen，Fan;Ruan，Lifang
水稻白叶枯病(Xanthomonas oryzaepv.oryzae，Xoo)是水稻白叶枯病的病原。病原菌具有多种转录调控因子，参与细胞过程的调控。在这里，我们确定了一个转录调控因子Gar(PXO_RS11965)，它参与调节Xoo的生长和毒力。值得注意的是，大蒜素X00的敲除增强了细菌对寄主水稻的毒力。序列分析和定量β-葡萄糖醛酸苷酶(GUS)分析表明，GAR正向调节σ54因子rpoN2的表达。进一步的实验证实，rpoN2的过表达恢复了由ga缺失引起的表型变化。我们的研究表明，GAR通过正向调节rpoN2的表达来影响细菌的生长和毒力。
Xanthomonas oryzaepv.oryzae(Xoo) is the causal agent of bacterial blight, one of the most devastating diseases of rice. Pathogenic bacteria possess numerous transcriptional regulators to participate in the regulation of cellular processes. Here, we identified a transcriptional regulator Gar (PXO_RS11965) that is involved in regulating the growth and virulence ofXoo. Notably, the knockout ofgarinXooenhanced bacterial virulence to the host rice. RNA-sequencing analysis and quantitative β-glucuronidase (GUS) assay indicated that Gar positively regulates the expression of a σ54factor rpoN2. Further experiments confirmed that overexpression ofrpoN2restored the phenotypic changes caused bygardeletion. Our research revealed that Gar influences bacterial growth and virulence by positively regulating the expression ofrpoN2.