A high-density integrated genetic linkage and radiation hybrid map of the laboratory rat.
A high-density integrated genetic linkage and radiation hybrid map of the laboratory rat.
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DOI：
10.1101/gr.9.6.ap1
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发表时间：
1999-05
期刊：
影响因子：
7
通讯作者：
R. Steen;A. Kwitek-Black;Christopher Glenn;J. Gullings-Handley;W. V. Etten;O. Atkinson;D. Appel;S. Twigger;Melanie Muir;Tim Mull;M. Granados;Mushira Kissebah;Kerri Russo;Robbin Crane;M. Popp;M. Peden;T. Matise;Donna M. Brown;Jian Lu;S. Kingsmore;P. Tonellato;S. Rozen;D. Slonim;Peter Young;M. Knoblauch;A. Provoost;Detlev Ganten;S. Colman;J. Rothberg;E. Lander;H. Jacob
中科院分区：
文献类型：
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作者：
R. Steen;A. Kwitek-Black;Christopher Glenn;J. Gullings-Handley;W. V. Etten;O. Atkinson;D. Appel;S. Twigger;Melanie Muir;Tim Mull;M. Granados;Mushira Kissebah;Kerri Russo;Robbin Crane;M. Popp;M. Peden;T. Matise;Donna M. Brown;Jian Lu;S. Kingsmore;P. Tonellato;S. Rozen;D. Slonim;Peter Young;M. Knoblauch;A. Provoost;Detlev Ganten;S. Colman;J. Rothberg;E. Lander;H. Jacob
实验鼠是生物医学研究的重要模型动物。然而，连接大鼠表型和基因型所需的遗传基础设施目前还不完整。在这里，我们报告了两个基因组图谱的构建和整合：大鼠的密集遗传连锁图谱和大鼠的第一个辐射杂交（RH）图谱。构建了SHRSP × BN和FHH × ACI两个杂交组合F2的遗传图谱，共包含4736个SSLP标记。4328个遗传标记的等位基因大小的特点是在48个最常用的近交系。RH图谱是lod >/= 3的框架图谱，包括983个SSLP，从而允许与各种遗传图谱上的标记以及与RH组上定位的标记整合。总的来说，这些图谱为>3000个基因和EST以及>8500个遗传标记（5211个我们的SSLP和>3500个由其他小组开发的SSLP）提供了综合参考。[Bihoreau等人（1997年）; James和Tanigami，RHdb（http：www.ebi.ac.uk/RHdb/index.html）;怀尔德（http：//www.nih.gov/niams/scientific/ratgbase）; Serikawa等人（1992年）; RATMAP服务器（http：ratmap.gen.gu.se）] RH地图（v.2.0）已张贴在我们的网站http://goliath.ifrc.mcw.edu/LGR/index.html或http://curatools.curagen.com/ratmap上。这两个网站都提供了一个RH映射服务器，研究人员可以在其中相对于该地图定位他们自己的RH向量。原始数据已存入RHdb数据库。总之，这些图谱为大鼠基因组学提供了基本工具。RH图谱提供了在大鼠基因组内快速定位遗传标记、基因和EST的方法。这些图谱为大鼠基因组学提供了基本工具。它们将促进多因子疾病和功能基因组学的研究，允许构建物理图谱，并为这一重要实验生物体的定向和大规模测序工作和比较基因组学提供支架。
The laboratory rat (Rattus norvegicus) is a key animal model for biomedical research. However, the genetic infrastructure required for connecting phenotype and genotype in the rat is currently incomplete. Here, we report the construction and integration of two genomic maps: a dense genetic linkage map of the rat and the first radiation hybrid (RH) map of the rat. The genetic map was constructed in two F2 intercrosses (SHRSP x BN and FHH x ACI), containing a total of 4736 simple sequence length polymorphism (SSLP) markers. Allele sizes for 4328 of the genetic markers were characterized in 48 of the most commonly used inbred strains. The RH map is a lod >/= 3 framework map, including 983 SSLPs, thereby allowing integration with markers on various genetic maps and with markers mapped on the RH panel. Together, the maps provide an integrated reference to >3000 genes and ESTs and >8500 genetic markers (5211 of our SSLPs and >3500 SSLPs developed by other groups). [Bihoreau et al. (1997); James and Tanigami, RHdb (http:www.ebi.ac.uk/RHdb/index.html); Wilder (http://www.nih.gov/niams/scientific/ratgbase); Serikawa et al. (1992); RATMAP server (http://ratmap.gen.gu.se)] RH maps (v. 2.0) have been posted on our web sites at http://goliath.ifrc.mcw.edu/LGR/index.html or http://curatools.curagen.com/ratmap. Both web sites provide an RH mapping server where investigators can localize their own RH vectors relative to this map. The raw data have been deposited in the RHdb database. Taken together, these maps provide the basic tools for rat genomics. The RH map provides the means to rapidly localize genetic markers, genes, and ESTs within the rat genome. These maps provide the basic tools for rat genomics. They will facilitate studies of multifactorial disease and functional genomics, allow construction of physical maps, and provide a scaffold for both directed and large-scale sequencing efforts and comparative genomics in this important experimental organism.