Application of non-lethal stable isotope analysis to assess feeding patterns of juvenile pallid sturgeon Scaphirhynchus albus: a comparison of tissue types and sample preservation methods.
Application of non-lethal stable isotope analysis to assess feeding patterns of juvenile pallid sturgeon Scaphirhynchus albus: a comparison of tissue types and sample preservation methods.
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DOI：
10.1111/j.1439-0426.2010.01527.x
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发表时间：
2010-12
影响因子：
0.9
通讯作者：
R. T. Andvik;Justin A. VanDeHey;M. Fincel;W. French;Katie N. Bertrand;S. Chipps;Robert A. Klumb;B. Graeb
中科院分区：
文献类型：
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作者：
R. T. Andvik;Justin A. VanDeHey;M. Fincel;W. French;Katie N. Bertrand;S. Chipps;Robert A. Klumb;B. Graeb
摘要 稳定同位素分析 (SIA) 的传统技术通常需要牺牲动物来收集组织样本。在研究苍白鲟鱼等濒危物种的饮食时，这可能会出现问题。我们的目标是 (i) 确定胸鳍组织（非致死）是否可以替代苍白鲟幼鱼 SIA 中的肌肉组织（致死），以及 (ii) 评估保存技术对稳定同位素值的影响。在实验室中，个体苍白鲟鱼幼鱼被饲养长达 186 天，并喂食摇蚊、鱼或市售的颗粒饲料。在鳍和肌肉组织之间观察到 δ15N 和 δ13C 存在显着的正相关关系 (r² ≥ 0.8)；在所有样本中，与肌肉组织相比，鳍中的同位素含量更高。使用稳定的 δ15N 和 δ13C 同位素，可以区分幼年苍白鲟鱼的摇蚊和以鱼为主的饮食对于快速生长的鱼（0.3 mm day−1）。冷冻和保存的鳍组织 δ15N 同位素强相关 (r2 = 0.89)，但 δ13C 同位素弱相关 (r2 = 0.16)。因此，建议冷冻保存鳍夹，以避免乙醇富集的混杂效应。这项研究证明了非致命技术在评估幼年苍白鲟鱼的时间综合饮食习惯方面的实用性，并且应该适用于其他受威胁或濒临灭绝的物种。
Summary Traditional techniques for stable isotope analysis (SIA) generally require sacrificing animals to collect tissue samples; this can be problematic when studying diets of endangered species such as the pallid sturgeon Scaphirhynchus albus. Our objectives were to (i) determine if pectoral fin tissue (non-lethal) could be a substitute for muscle tissue (lethal) in SIA of juvenile pallid sturgeon, and (ii) evaluate the influence of preservation techniques on stable isotope values. In the laboratory, individual juvenile pallid sturgeon were held for up to 186 day and fed chironomids, fish, or a commercially available pellet diet. Significant, positive relationships (r² ≥ 0.8) were observed between fin and muscle tissues for both δ15N and δ13C; in all samples isotopes were enriched in fins compared to muscle tissue. Chironomid and fish based diets of juvenile pallid sturgeon were distinguishable for fast growing fish (0.3 mm day−1) using stable δ15N and δ13C isotopes. Frozen and preserved fin tissue δ15N isotopes were strongly related (r2 = 0.89) but δ13C isotopes were weakly related (r2 = 0.16). Therefore, freezing is recommended for preservation of fin clips to avoid the confounding effect of enrichment by ethanol. This study demonstrates the utility of a non-lethal technique to assess time integrated food habits of juvenile pallid sturgeon and should be applicable to other threatened or endangered species.