Alteration pattern of tear cytokines during the course of a day: diurnal rhythm analyzed by multicytokine assay.
Alteration pattern of tear cytokines during the course of a day: diurnal rhythm analyzed by multicytokine assay.
复制标题
DOI：
10.1016/j.cyto.2005.11.013
复制
发表时间：
2006-01
期刊：
影响因子：
3.8
通讯作者：
E. Uchino;S. Sonoda;N. Kinukawa;T. Sakamoto
中科院分区：
文献类型：
--
作者：
E. Uchino;S. Sonoda;N. Kinukawa;T. Sakamoto
我们的目的是研究炎性泪液细胞因子在一天中的变化模式和相互作用。采用前瞻性实验设计，从2004年4月至2005年3月期间收集了28名健康志愿者的泪液。在9：00、12：00、16：00、21：00和24：00 h，通过毛细血管流出从每只眼收集泪液（10μl）。使用流式细胞仪微珠阵列测量炎性细胞因子IL-1β、IL-6、IL-8、IL-10、IL-12 p70和TNF-α的浓度。虽然泪液细胞因子的浓度在眼睛之间变化很大，但在一天的过程中，细胞因子的量在每只眼睛中具有特定的变化模式。IL-1β、IL-6、IL-10、IL-12 p70和TNF-α在早晨和深夜显示轻微升高。IL-8全天保持低水平。IL-8与TNF-α、IL-12 p70的变化有显著性差异（P<0.01）。每种促炎细胞因子与抗炎细胞因子IL-10的比率在一天中没有显著变化。泪液中细胞因子的含量在白天有一定的变化规律。这种昼夜节律可能会影响一天中眼表疾病的症状。
Our aim was to study the alteration pattern and interaction of inflammatory tear cytokines during the course of a day. Using a prospective, experimental design, tears were collected from 28 healthy volunteers with normal eyes during the period from April 2004 to March 2005. Tears (10μl) were collected by capillary outflow from each eye at 9:00, 12:00, 16:00, 21:00, and 24:00h. The concentrations of inflammatory cytokines, IL-1β, IL-6, IL-8, IL-10, IL-12p70, and TNF-α were measured using cytometric bead arrays. Although the concentration of tear cytokines varied widely among eyes, the amount of cytokine had a specific alteration pattern in each eye during the course of a day. IL-1β, IL-6, IL-10, IL-12p70, and TNF-α showed slight increases in the morning and the late evening. IL-8 remained low throughout the day. The alteration pattern of IL-8 was significantly different from those of TNF-α and IL-12p70 (P<0.01). The ratio of each pro-inflammatory cytokine to anti-inflammatory cytokine IL-10 did not significantly change throughout the day. The amount of tear cytokines changed during daytime with a specific pattern. This diurnal rhythm may influence symptoms of ocular surface diseases during the course of a day.