Extensive antigenic changes in an atypical isolate of very virulent infectious bursal disease virus and experimental clinical control of this virus with an antigenically classical live vaccine
Extensive antigenic changes in an atypical isolate of very virulent infectious bursal disease virus and experimental clinical control of this virus with an antigenically classical live vaccine
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DOI：
10.1080/03079450410001724049
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发表时间：
2004-08-01
期刊：
影响因子：
2.8
通讯作者：
Borne, PM
中科院分区：
文献类型：
--
作者：
Eterradossi, N;Gauthier, C;Borne, PM
埃及99323株传染性法氏囊病病毒分离株是在一次急性传染性法氏囊病病例的国际调查中发现的。其独特的抗原性特征是在抗原捕获酶联免疫吸附试验中，中和单抗3、4、5、6、8和9的结合显著减少。对99323中编码VP2主要免疫原域的基因组区域的核苷酸序列分析显示，在抗原性关键位置发生了氨基酸变化，但系统发育分析表明99323与典型的强毒IBDV(如89163分离株)有关。实验研究了抗原性经典传染性法氏囊病活疫苗对99323或89163的无特定病原体的鸡的保护作用。根据临床体征、生长迟缓、法氏囊与体重的比率以及攻击后法氏囊的组织学损害，这两种病毒都得到了类似的控制。这些结果证明，对经典活体抗原的主动抗体反应可以在临床上控制抗原性非典型、非常毒力的IBDV的感染。
The 99323 Egyptian isolate of infectious bursal disease (IBD) virus (IBDV) was identified during an international survey of acute IBD cases. Its unique antigenicity was characterized by a markedly reduced binding of neutralizing monoclonal antibodies 3, 4, 5, 6, 8 and 9 in an antigen-capture enzyme-linked immunosorbent assay. Nucleotide sequencing of the genome region encoding the VP2 major immunogenic domain in 99323 revealed amino acid changes occurring at positions critical for antigenicity, but phylogenetic analysis demonstrated that 99323 was related to typical, very virulent IBDV (e.g. isolate 89163). Protection experimentally afforded by an antigenically classical live IBD vaccine was investigated in specific pathogen free chickens challenged with 99323 or 89163. Both viruses were similarly controlled, as evaluated by clinical signs, growth retardation, bursa-to-body weight ratios and histological lesions of the bursa after challenge. These results document that an active antibody response to a classical live antigen may clinically control infection by an antigenically atypical very virulent IBDV.