In situ analysis of hepatitis B virus (HBV) antigen and DNA in HBV-induced hepatocellular carcinoma.
In situ analysis of hepatitis B virus (HBV) antigen and DNA in HBV-induced hepatocellular carcinoma.
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乙型肝炎病毒 (HBV) 抗原和 DNA 在 HBV 诱导的肝细胞癌中的原位分析
DOI：
10.1186/s13000-022-01194-8
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发表时间：
2022-01-16
影响因子：
2.6
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Zheng Y;Xu M;Zeng D;Tong H;Shi Y;Feng Y;Zhang X
B型肝炎病毒（HBV）感染是东亚地区肝细胞癌（HCC）的主要危险因素。本研究的目的是在组织学水平上进一步研究HBV相关的HCC及其周围组织中病毒抗原和DNA的丰度。除常规组织病理学检查外，还对131例HBsAg阳性的HCC患者行肝切除术后的组织进行了HBV DNA原位杂交（ISH）和HBsAg免疫组化（IHC）。同时测定血清甲胎蛋白、基础生化及免疫学指标。HBV DNA原位杂交和HBsAg免疫组化阳性率分别为31.3%和92.9%（P < 0.0001）。这两种标志物之间的相关性水平在肿瘤中（p < 0.0001）比在肿瘤周围组织中（p = 0.01）显著得多。癌旁组织HBsAg阳性率为86.6%，明显高于癌组织（29.9%，P < 0.0001），两组间有显著性差异（P <0.001）。而肿瘤组织和癌旁组织中HBV DNA原位杂交的阳性率相当（17.6%vs22.9%，p = 0.36）。然而，肿瘤组织中的HBV DNA信号显示主要核定位（87.0%），而邻近组织中的染色模式是混合的（43.3%核定位，p = 0.0015）。最后，肿瘤内HBV DNA/HBsAg阳性与主要组织学标志物（微血管浸润、肿瘤分化等）之间无显著相关性。或术后复发。这些数据证实了HBV DNA的大部分整合状态，与周围组织相比，肿瘤中表面抗原的表达较弱且定位改变。HBsAg和HBVDNA的显著不同的流行率和定位反映了导致HBV诱导的肿瘤发生的复杂和异质性机制。在线版本包含补充材料，可通过10.1186/s13000-022-01194-8获取。
Hepatitis B Virus (HBV) infection is the major risk factor for hepatocellular carcinoma (HCC) in East Asia. Here we aimed to further investigate the abundance of viral antigen and DNA within HBV-related HCC and surrounding tissues at histological level. In addition to routine histopathology, in situ hybridization (ISH) of HBV DNA and immunohistochemistry (IHC) of HBsAg were performed in tissues from 131 HBsAg-positive HCC patients undergoing liver resection. Serum α-fetoprotein together with basic biochemical and immunological parameter was also measured. Overall, the ISH of HBV DNA and IHC of HBsAg showed 31.3% and 92.9% positive rate respectively (p < 0.0001). The level of correlation between these two markers was much more significant in tumor (p < 0.0001) than in tumor-surrounding tissue (p = 0.01). HBsAg exhibited a much higher positive rate in tumor-adjacent tissue than in tumor tissue (86.6% versus 29.9%, p < 0.0001) with significantly different staining pattern. By contrast, the positive rate of HBV DNA ISH was comparable in tumor and surrounding tissue (17.6% versus 22.9%, p = 0.36). Yet the HBV DNA signal in tumor tissue showed predominant nuclear localization (87.0%) whereas staining pattern in adjacent tissue was mixed (43.3% nuclear localization, p = 0.0015). Finally, no significant association between intra-tumor HBV DNA/HBsAg positivity and major histological markers (microvascular invasion, tumor differentiation, etc.) or recurrence after surgery was observed. These data confirmed the largely integrated state of HBV DNA, weaker expression and altered localization of surface antigen in tumor compared with surrounding tissue. The strikingly different prevalence and localization of HBsAg and HBV DNA reflected the complex and heterogeneous mechanisms leading to HBV-induced tumorigenesis. The online version contains supplementary material available at 10.1186/s13000-022-01194-8.
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影响因子：
64.8
作者：
DEJEAN, A;BOUGUELERET, L;TIOLLAIS, P
通讯作者：
TIOLLAIS, P
影响因子：
30.8
作者：
Sung, Wing-Kin;Zheng, Hancheng;Luk, John M.
通讯作者：
Luk, John M.
影响因子：
29.4
作者：
Tan, Anthony Tanoto;Yang, Ninghan;Bertoletti, Antonio
通讯作者：
Bertoletti, Antonio
HBV DNA 原位测定的评估 慢性乙型肝炎的观察性真实世界研究
影响因子：
1.6
作者：
Li C;Zhang W;Shi B;Chen G;Zheng Y;An Y;Sun M;Feng Y;Shang Q;Zhang X
通讯作者：
Zhang X
肝细胞癌中 HBV 整合的基因组和致癌偏好
影响因子：
16.6
作者：
Zhao LH;Liu X;Yan HX;Li WY;Zeng X;Yang Y;Zhao J;Liu SP;Zhuang XH;Lin C;Qin CJ;Zhao Y;Pan ZY;Huang G;Liu H;Zhang J;Wang RY;Yang Y;Wen W;Lv GS;Zhang HL;Wu H;Huang S;Wang MD;Tang L;Cao HZ;Wang L;Lee TL;Jiang H;Tan YX;Yuan SX;Hou GJ;Tao QF;Xu QG;Zhang XQ;Wu MC;Xu X;Wang J;Yang HM;Zhou WP;Wang HY
通讯作者：
Wang HY