Diurnal rhythms in ornithine decarboxylase activity and norepinephrine and acetylcholine synthesis in submaxillary lymph nodes and spleen of young and aged rats during Freund's adjuvant-induced arthritis
Diurnal rhythms in ornithine decarboxylase activity and norepinephrine and acetylcholine synthesis in submaxillary lymph nodes and spleen of young and aged rats during Freund's adjuvant-induced arthritis
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DOI：
10.1016/s0006-8993(98)00015-8
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发表时间：
1998-04-13
期刊：
影响因子：
2.9
通讯作者：
Esquifino, AI
中科院分区：
文献类型：
--
作者：
Cardinali, DP;Brusco, LI;Esquifino, AI
衰老与许多昼夜节律的幅度衰减和周期变化有关。本研究观察了青年(50日龄)和老年(18月龄)大鼠在弗氏佐剂诱导的关节炎过程中，淋巴结和脾的24小时细胞增殖节律(通过测定鸟氨酸脱羧酶活性)以及突触前肾上腺素能和胆碱能标志物的变化。前一天，以及注射弗氏佐剂后的第6、12和18天，对幼年和老年SD大鼠进行了研究。在佐剂注射后第16天，老年大鼠的后爪炎症比青年大鼠轻，主要是在脚踝关节。淋巴和脾的鸟氨酸脱羧酶活性在24小时内有明显的变化，在一天中活性最高。治疗前，老年大鼠两种组织中的酶活性值均明显低于治疗前。在免疫反应中，淋巴结和脾鸟氨酸脱羧酶增加了8-10倍，随着关节炎的发展，每日变化的幅度逐渐减小。在所有病例中，鸟氨酸脱羧酶活性的中位数和波幅在老年大鼠中最低。颌下淋巴结和脾酪氨酸羟基酶活性在夜间达到最大值。在注射分支杆菌佐剂后的各个时间间隔，老年大鼠酪氨酸羟化酶活性节律的波幅和中值最低。颌下淋巴结H-3-乙酰胆碱合成高峰出现在下午。在弗氏佐剂注射后第6天和第12天，老年大鼠淋巴结H-3-乙酰胆碱的合成明显减少。用佐剂载体治疗的大鼠颌下淋巴结或脾鸟氨酸脱羧酶和酪氨酸羟基酶活性，或颌下淋巴结H-3-乙酰胆碱合成的昼夜差异与未治疗对照组没有显著差异。这一结果与免疫介导性炎症反应的年龄相关性下降是一致的。中枢昼夜节律振荡器的活动部分通过自主神经系统被驱动到器官，在衰老过程中似乎也会恶化。(C)1998年爱思唯尔科学公司。
Aging has been associated with attenuation of amplitude and changes in period of many circadian rhythms. The present study was carried out to examine, in young (50 days old) and old (18 months old) rats, whether 24-h rhythms of cell proliferation (as assessed by measuring ornithine decarboxylase activity) and of presynaptic adrenergic and cholinergic markers change in lymph nodes and spleen during Freund's adjuvant-induced arthritis. Groups of young and old Sprague-Dawley rats were studied the day before, and on days 6, 12 and 18 after Freund's adjuvant injection. On day 16 after adjuvant injection, inflammation of hind paws, mainly in the ankle joints, was less marked in old than in young rats. Lymph node and splenic ornithine decarboxylase activity exhibited significant 24-h variations with maximal activity during daily hours. Before treatment, enzyme activity values were significantly lower in old rats in both tissues examined. During the immune reaction, lymph node and splenic ornithine decarboxylase augmented 8-10-fold, with progressively smaller amplitude of daily variations as arthritis developed. In every case, mesor and amplitude of ornithine decarboxylase activity were lowest in old rats. Submaxillary lymph node and splenic tyrosine hydroxylase activity attained maximal values at night. At every time interval after mycobacterium adjuvant injection, amplitude and mesor of tyrosine hydroxylase activity rhythm were lowest in old rats. A maximum in submaxillary lymph node H-3-acetylcholine synthesis occurred at the afternoon. On day 6 and 12 after Freund's adjuvant injection, lymph node H-3-acetylcholine synthesis was significantly smaller in old rats. Day-night differences in submaxillary lymph node or splenic ornithine decarboxylase and tyrosine hydroxylase activities, or in submaxillary lymph node H-3-acetylcholine synthesis, of rats treated with the adjuvant's vehicle, did not differ significantly from those seen in untreated controls. The results are compatible with an age-dependent decline of immune-mediated inflammatory responses. The activity of the central circadian oscillator, driven to the organs in part via the autonomic nervous system, seems also to deteriorate during aging. (C) 1998 Elsevier Science B.V.