Osteogenic differentiation of mesenchymal stem cells (MSCs) induced by three calcium phosphate ceramic (CaP) powders: A comparative study
Osteogenic differentiation of mesenchymal stem cells (MSCs) induced by three calcium phosphate ceramic (CaP) powders: A comparative study
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三种磷酸钙陶瓷（CaP）粉末诱导间充质干细胞（MSC）成骨分化的比较研究
DOI：
10.1016/j.msec.2017.05.145
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发表时间：
2017-11-01
影响因子：
7.9
通讯作者：
Zhao, Jinmin
中科院分区：
文献类型：
--
作者：
Li, Yuquan;Jiang, Tongmeng;Zhao, Jinmin
磷酸钙陶瓷（CaPs）由于其与骨的相似性而被认为是骨移植的合适替代品。磷酸三钙（TCP）、羟基磷灰石（HA）和双相磷酸钙（BCP）是最常用的cap。本研究利用骨髓间充质干细胞（MSCs）对HA、TCP和BCP三种cap （HA/ β -TCP重量比为60/40）粉末的成骨能力进行了研究和比较。培养7、14、21天后，测定MSCs成骨细胞的形成、矿化和成骨标志物的基因表达。结果显示，与对照组相比，CaPs粉末对MSCs具有正向的成骨分化作用，其表现为碱性磷酸酶（ALP）活性升高，ALP和茜素红S染色阳性，成骨基因表达上调。在三种cap中，BCP粉末的成骨效果最为显著。BCP旁边是TCP。本研究支持BCP在临床修复骨缺损方面具有较好的应用前景。(C) 2017 Elsevier B.V.版权所有
Calcium phosphate ceramics (CaPs) appear to be suitable alternatives for bone grafts because of their similarity to bone. Tricalcium phosphate (TCP), hydroxyapatite (HA) and biphasic calcium phosphates (BCP) are most commonly used CaPs. In this study, we studied and compared the osteoinductive ability of the powders of the three CaPs including HA, TCP and BCP (HA/beta-TCP weight ratio of 60/40) by using bone marrow derived mesenchymal stem cells (MSCs). Osteoblastic formation, mineralization and gene expression of osteogenesis markers were measured in MSCs after 7, 14, 21 days of culture. Results showed a positive osteogenic differentiation effect of CaPs powders on MSCs as evidenced by an increased alkaline phosphatase (ALP) activity, positive ALP and Alizarin Red S staining, and upregulated osteoblastic gene expression compared with control. For the three CaPs, BCP powders showed the most prominent effect on osteoinduction. Next to BCP is TCP. Thus, this study supported that BCP may have better application prospects for repairing of bone defects in clinic. (C) 2017 Elsevier B.V. All rights reserved.