CONFORMATIONAL ALTERATION OF MESSENGER-RNA STRUCTURE AND THE POSTTRANSCRIPTIONAL REGULATION OF ERYTHROMYCIN-INDUCED DRUG-RESISTANCE
CONFORMATIONAL ALTERATION OF MESSENGER-RNA STRUCTURE AND THE POSTTRANSCRIPTIONAL REGULATION OF ERYTHROMYCIN-INDUCED DRUG-RESISTANCE
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DOI：
10.1093/nar/8.24.6081
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发表时间：
1980-01-01
影响因子：
14.9
通讯作者：
DUBNAU, D
中科院分区：
文献类型：
--
作者：
GRYCZAN, TJ;GRANDI, G;DUBNAU, D
给出了质粒pE194的ermcgene的DNA序列。这一决定因素是红霉素诱导的对大环内酯-林可胺-链霉素B组抗生素耐药的原因，并指定了一种29,000道尔顿诱导蛋白。它们的ermcpromoter的位置，以及可能的转录终止子的位置，都是通过序列和转录作图确定的。该序列包含一个开放阅读框，足以编码先前鉴定的29,000 daltonermCprotein。在启动子和假定的ATG起始密码子之间是一个141个碱基对的先导序列，在这个序列中，一些调节（组成）突变已经被定位和测序。引线具有第二开放阅读框，足以编码19个氨基酸的肽。这表明红霉素的诱导涉及到核糖体结合的mRNA构象之间的转换，因此在诱导剂的存在下，核糖体结合序列和合成29K蛋白的起始密码子被揭露。提出了可能的先导序列的活性和非活性折叠构型，以及对这些构型的调控突变的影响。
The DNA sequence of theermCgene of plasmid pE194 is presented. This determinant is responsible for erythromycin-induced resistance to the macrolide-lincosamide-streptogramin B group of antibiotics and specifies a 29,000 dalton inducible protein. The locations of theermCpromoter, as well as that of a probable transcriptional terminator, are established both from the sequence and by transcription mapping. The sequence contains an open reading frame sufficient to encode the previously identified 29,000 daltonermCprotein. Between the promoter and the putative ATG start codon is a 141 base pair leader sequence, within which several regulatory (constitutive) mutations have been mapped and sequenced. The leader has a second open reading frame, sufficient to encode a 19 amino acid peptide. It is suggested that induction by erythromycin involves a shift between alternative ribosome-bound mRNA conformations, so that the ribosome binding sequence and the start codon for synthesis of the 29K protein are unmasked in the presence of inducer. Possible active and inactive folded configurations of the leader sequence are presented, as well as the effects on these configurations of regulatory mutations.