Protein sequence of endothelial glycoprotein IIIa derived from a cDNA clone. Identity with platelet glycoprotein IIIa and similarity to "integrin".
Protein sequence of endothelial glycoprotein IIIa derived from a cDNA clone. Identity with platelet glycoprotein IIIa and similarity to "integrin".
复制标题
DOI：
10.1016/s0021-9258(18)61290-6
复制
发表时间：
1987-03
期刊：
影响因子：
--
通讯作者：
L. Fitzgerald;B. Steiner;S. C. Rall;S. Lo;D. Phillips
中科院分区：
文献类型：
--
作者：
L. Fitzgerald;B. Steiner;S. C. Rall;S. Lo;D. Phillips
血小板膜糖蛋白（GP）IIIa与GP IIb形成Ca 2+依赖性异二聚体复合物。GP IIb-IIIa复合物构成刺激血小板上的纤维蛋白原和纤连蛋白受体。一种生物化学和免疫学上相似的膜糖蛋白复合物存在于内皮细胞上。人脐静脉内皮细胞cDNA文库筛选使用寡核苷酸探针设计从血小板GP IIIa获得的肽序列。对cDNA克隆进行测序，发现其编码84.5 kDa的蛋白质。翻译的内皮cDNA包含5个序列，对应于血小板GP IIIa中的肽序列，包括氨基末端19个残基。因此，GP IIIa的内皮和血小板形式显然是相同的。糖蛋白IIIa由一个长的氨基末端胞外结构域、一个29个残基的疏水性跨膜片段和一个短的羧基末端胞质结构域组成，该结构域具有几个潜在的N-连接糖基化位点和四个富含半胱氨酸的串联重复序列。糖蛋白IIIa与“整合素”（一种来自鸡胚成纤维细胞的纤连蛋白受体）具有47%的氨基酸序列同源性。这种同源性表明GP IIIa是细胞表面粘附受体家族的成员。
Platelet membrane glycoprotein (GP) IIIa forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets. A biochemically and immunologically similar membrane glycoprotein complex is present on endothelial cells. A human umbilical vein endothelial cell cDNA library was screened using oligonucleotide probes designed from peptide sequences obtained from platelet GP IIIa. A cDNA clone was sequenced and found to encode a protein of 84.5 kDa. The translated endothelial cDNA contained five sequences that corresponded to peptide sequences in platelet GP IIIa, including the amino-terminal 19 residues. Thus, the endothelial and platelet forms of GP IIIa are apparently identical. Glycoprotein IIIa consists of a long amino-terminal extracellular domain with several potential N-linked glycosylation sites and four cysteine-rich tandem repeats, a 29-residue hydrophobic transmembrane segment, and a short carboxyl-terminal cytoplasmic domain. Glycoprotein IIIa has a 47% amino acid sequence homology to “integrin,” a fibronectin receptor from chicken embryo fibroblasts. This homology suggests that GP IIIa is a member of a family of cell-surface adhesion receptors.