The 70‐kDa major DNA‐compacting protein of the chloroplast nucleoid is sulfite reductase
The 70‐kDa major DNA‐compacting protein of the chloroplast nucleoid is sulfite reductase
复制标题
叶绿体核的 70 kDa 主要 DNA 压缩蛋白是亚硫酸盐还原酶
DOI：
10.1016/s0014-5793(00)02342-5
复制
发表时间：
2001
期刊：
影响因子：
3.5
通讯作者：
T. Hase
中科院分区：
文献类型：
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作者：
N. Sato;M. Nakayama;T. Hase
叶绿体类核是叶绿体DNA和各种蛋白质的复合物。豌豆叶绿体类核蛋白中含有丰富的70-kDa蛋白质，经N-末端序列分析、免疫印迹分析、分光光度法和酶活性分析，鉴定为亚硫酸盐还原酶。重组玉米亚硫酸盐还原酶确实能够在体外压缩叶绿体DNA并形成类核颗粒。亚硫酸盐还原酶的结构组织的类核的作用进行了讨论。
The chloroplast nucleoid is a complex of chloroplast DNA and various, mostly uncharacterized proteins. An abundant 70-kDa protein of the isolated nucleoids of pea chloroplasts was identified as sulfite reductase by N-terminal sequence analysis as well as immunoblot analysis, spectrophotometry and enzyme activity analysis. Recombinant maize sulfite reductase was indeed able to compact chloroplast DNA and to form nucleoid-like particles in vitro. The role of sulfite reductase in the structural organization of the nucleoid is discussed.
Nemoto, Y.、S. Kawano、T. Nagata
DOI：
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发表时间：
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影响因子：
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