Methylation status of putative differentially methylated regions of porcine IGF2 and H19
Methylation status of putative differentially methylated regions of porcine IGF2 and H19
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DOI：
10.1002/mrd.20802
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发表时间：
2008-05-01
影响因子：
2.5
通讯作者：
Lee, Hoon Taek
中科院分区：
文献类型：
--
作者：
Han, Dong Wook;Im, Young Bin;Lee, Hoon Taek
本研究旨在确定猪印迹基因胰岛素样生长因子2和H19(IGF2-H19)的差异甲基化区域，并通过鉴定这些区域在生殖细胞、猪胚胎组织、成年猪以及体细胞核移植克隆后代中的甲基化模式来评估IGF2-H19的基因组印迹状态。猪IGF2-H19DMR表现出正常的单等位基因甲基化模式(即父系或母系衍生的等位基因甲基化)，类似于在人类和小鼠基因组中观察到的相同基因的模式。对IGF2-H19DMRS甲基化模式的检测表明，锌指蛋白结合位点CTCF1和2在对照和克隆后代中都没有表现出差异甲基化。相反，IGF2基因的CTCF3和DMR2基因座在克隆后代中显示异常甲基化，而在对照后代和成年猪的组织中显示正常的差异或中等甲基化模式。因此，我们的数据表明，猪IGF2-H19基因座的基因组印记调控在物种之间是保守的，印记基因调控元件中的异常甲基化模式可能导致成功重编程所需基因的协调表达的改变，从而可能导致核移植导致的猪基因组重编程效率低下。是Reprod。戴夫。75：777-784,2008。(C)2008年Wiley-Liss，Inc.
This study was designed to identify the putative differentially methylated regions (DMRs) of the porcine imprinted genes insulin-like growth factor 2 and H19 (IGF2-H19), and to assess the genomic imprinting status of IGF2-Hl9 by identifying the methylation patterns of these regions in germ cells, and in tissues from porcine fetuses, an adult pig, as well as cloned offspring produced by somatic cell nuclear transfer (SCNT). Porcine IGF2-Hl9 DMRs exhibit a normal monoallelic methylation pattern (i.e., either the paternally- or the maternally derived allele is methylated) similar to the pattern observed for the same genes in the human and mice genomes. Examination of the methylation patterns of the IGF2-H19 DMRs revealed that the zinc finger protein binding sites CTCF1 and 2 did not exhibit differential methylation in both control and cloned offspring. In contrast, the CTCF3 and DMR2 loci of the IGF2 gene showed abnormal methylation in cloned offspring, but a normal differential or moderate methylation pattern in tissues from control offspring and an adult pig. Our data thus suggest that regulation of genomic imprinting at the porcine IGF2-H19 loci is conserved among species, and that the abnormal methylation pattern in the regulatory elements of imprinted genes may lead to an alteration in the coordinated expression of genes required for successful reprogramming, which, in consequence, may contribute to the low efficiency of porcine genome reprogramming induced by nuclear transfer, Mol. Reprod. Dev. 75: 777-784, 2008. (C) 2008 Wiley-Liss, Inc.