Cryocooling and radiation damage in macromolecular crystallography
Cryocooling and radiation damage in macromolecular crystallography
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DOI：
10.1107/s0907444905034207
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发表时间：
2006-01-01
影响因子：
2.2
通讯作者：
Owen, RL
中科院分区：
文献类型：
--
作者：
Garman, EF;Owen, RL
用于大分子结晶学的结晶学技术的进步与减少在收集衍射数据期间造成的X射线损伤的有害影响的努力紧密地交织在一起。简要概述了冷冻方法及其基本原理。然后总结了我们目前对低温晶体中辐射损伤的有限了解，旨在将其影响降至最低的研究，最后是一些实际上利用它来分相和扩展结构知识的发展。
Advances in cryocrystallographic techniques for macromolecular crystallography have been intimately intertwined with efforts to reduce the deleterious effects of X-ray damage inflicted during the collection of diffraction data. A brief overview of cryomethods and their rationale is given. This is followed by a summary of our current limited understanding of radiation damage in cryocooled crystals, investigations aimed at minimizing its effects and finally some developments which actually utilize it both for phasing and to extend structural knowledge.