Expression at the cell surface of biologically active fusion and hemagglutinin/neuraminidase proteins of the paramyxovirus simian virus 5 from cloned cDNA.
Expression at the cell surface of biologically active fusion and hemagglutinin/neuraminidase proteins of the paramyxovirus simian virus 5 from cloned cDNA.
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来自克隆 cDNA 的副粘病毒猿病毒 5 的生物活性融合蛋白和血凝素/神经氨酸酶蛋白在细胞表面的表达。
DOI：
10.1073/pnas.82.22.7520
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发表时间：
1985
影响因子：
11.1
通讯作者：
Lamb,RA
中科院分区：
文献类型：
--
作者：
Paterson,RG;Hiebert,SW;Lamb,RA
将编码副粘病毒猿猴病毒5的融合蛋白（F）和血凝素/神经氨酸酶蛋白（HN）的mRNA的cDNA插入猿猴病毒40晚期启动子控制下的真核表达载体中。在重组感染细胞中合成的F和HN蛋白在电泳迁移率和糖基化方面与在猴病毒5感染细胞中合成的蛋白质没有区别。此外，表达的F和HN蛋白已被证明是锚定在质膜中的间接活细胞免疫荧光，F介导的合胞体的形成，和HN的能力，导致红细胞的红细胞吸附到感染的细胞表面的生物活性形式。
cDNAs encoding the mRNAs for the fusion protein (F) and the hemagglutinin/neuraminidase protein (HN) of the paramyxovirus simian virus 5 have been inserted into a eukaryotic expression vector under the control of the simian virus 40 late promoter. The F and HN proteins synthesized in recombinant infected cells are indistinguishable in terms of electrophoretic mobility and glycosylation from the proteins synthesized in simian virus 5-infected cells. In addition, the expressed F and HN proteins have been shown to be anchored in the plasma membrane in a biologically active form by indirect live cell immunofluorescence, the F-mediated formation of syncytia, and the ability of HN to cause the hemadsorption of erythrocytes to the infected cell surface.