Correlations of the expression of vascular endothelial growth factor B and its isoforms in hepatocellular carcinoma with clinico-pathological parameters
Correlations of the expression of vascular endothelial growth factor B and its isoforms in hepatocellular carcinoma with clinico-pathological parameters
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DOI：
10.1002/jso.21095
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发表时间：
2008-09-01
影响因子：
2.5
通讯作者：
Nakao, Akimasa
中科院分区：
文献类型：
--
作者：
Kanda, Mitsuro;Nomoto, Shuji;Nakao, Akimasa
背景：血管内皮生长因子（VEGF）通过血管生成参与癌细胞的生长。目前VEGF-B的作用尚未完全阐明。我们研究了肝细胞癌(HCC)中VEGF-B及其形式VEGF-B167和VEGF-B186的选择性剪接表达与病理结果和预后的相关性。方法：对48例HCC患者进行了研究。我们使用定量实时逆转录聚合酶链反应（RT-PCR）分析检测了原发性HCC和非癌组织中总VEGF-B、VEGF-B167和VEGF-B186的mRNA表达。结果：在48个HCC中的16个（33.3%）中，与相应的非癌性肝组织相比，总VEGF-B的表达增加。关于亚型，VEGF-B167 和 VEGF-B186 的表达分别在 48 例 HCC 中的 17 例（35.4%）和 48 例 HCC 中的 33 例（68.75%）中升高。 HCC中总VEGF-B高表达的病例与病理分期晚期（P < 0.018）、肿瘤多重性（P < 0.033）、血管侵犯（P < 0.045）和包膜缺乏形成（P < 0.027）显着相关。 VEGF-B167 的结果与总 VEGF-B 相似。结论：我们的结果表明 VEGF-B 的表达与 HCC 中的肿瘤生长和侵袭性相关。 VEGF-B167 似乎是临床上占主导地位的亚型。
Background: The vascular endothelial growth factor (VEGF) is involved in the growth of cancer cells through angiogenesis. At present the role of VEGF-B has not been clarified completely. We investigated correlations of the expression of VEGF-B and its is forms, VEGF-B167 and VEGF-B186, by alternative splicing in hepatocellular carcinoma (HCC) with the pathological findings and prognosis.Methods: Forty-eight patients with HCC were investigated. We examined the mRNA expression of total VEGF-B, VEGF-B167 and VEGF-B186 in primary HCC and non-cancerous tissues using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) analysis.Results: In 16 (33.3%) of 48 HCCs, the expression of total VEGF-B increased compared with the corresponding non-cancerous liver tissues. Regarding the isoforms, the expression of VEGF-B167 and VEGF-B186 was increased in 17 (35.4%) of 48 and 33 (68.75%) of 48 HCCs, respectively. Cases with high expression level of total VEGF-B in HCC significantly correlated with the advanced pathological stage (P < 0.018), tumor multiplicity (P < 0.033), vascular invasion (P < 0.045) and lack of capsule formation (P < 0.027). The result in VEGF-B167 was similar to total VEGF-B.Conclusions: Our results indicated that the expression of VEGF-B is correlated with tumor growth and invasiveness in HCC. VEGF-B167 seemed to be the clinically dominant isoform.