Genetics and Pathogenesis of Feline Infectious Peritonitis Virus
Genetics and Pathogenesis of Feline Infectious Peritonitis Virus
复制标题
DOI：
10.3201/eid1509.081573
复制
发表时间：
2009-09-01
影响因子：
11.8
通讯作者：
O'Brien, Stephen J.
中科院分区：
文献类型：
--
作者：
Brown, Meredith A.;Troyer, Jennifer L.;O'Brien, Stephen J.
猫冠状病毒（FCoV）是野猫种群和猫群中的地方病，经常在致命的猫传染性腹膜炎（FIP）爆发之前爆发。FCoV具有2种生物型：致病性疾病和猫肠道冠状病毒（FECV）的良性感染。关于遗传上独特的无毒和毒性形式是否共存或无毒形式是否在体内突变，导致FIR仍存在不确定性。为了解决这些替代假设，我们从临床上健康和患病的FCoV感染的猫（8例FIP病例和48只FECV无症状动物）中分离出病毒序列;从4个基因片段中产生735个序列，并进行系统发育分析。根据膜和非结构蛋白7 b基因中观察到的遗传距离，健康猫的病毒序列与患病猫的病毒序列不同。这些数据表明，不同的循环毒力和无毒力菌株在自然人群中。此外，5个膜蛋白氨基酸残基的功能潜力区分健康的猫与猫FIR这些发现可能有潜力作为诊断标志物的烈性FIP相关的FCoV。
Feline coronavirus (FCoV) is endemic in feral cat populations and cat colonies, frequently preceding outbreaks of fatal feline infectious peritonitis (FIP). FCoV exhibits 2 biotypes: the pathogenic disease and a benign infection with feline enteric coronavirus (FECV). Uncertainty remains regarding whether genetically distinctive avirulent and virulent forms coexist or whether an avirulent form mutates in vivo, causing FIR To resolve these alternative hypotheses, we isolated viral sequences from FCoV-infected clinically healthy and sick cats (8 FIP cases and 48 FECV-asymptomatic animals); 735 sequences from 4 gene segments were generated and subjected to phylogenetic analyses. Viral sequences from healthy cats were distinct from sick cats on the basis of genetic distances observed in the membrane and nonstructural protein 7b genes. These data demonstrate distinctive circulating virulent and avirulent strains in natural populations. In addition, 5 membrane protein amino acid residues with functional potential differentiated healthy cats from cats with FIR These findings may have potential as diagnostic markers for virulent FIP-associated FCoV.