Electrochemical fecal pellet sensor for simultaneous real-time ex vivo detection of colonic serotonin signalling and motility.
Electrochemical fecal pellet sensor for simultaneous real-time ex vivo detection of colonic serotonin signalling and motility.
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DOI：
10.1038/srep23442
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发表时间：
2016-03-22
影响因子：
4.6
通讯作者：
Patel BA
中科院分区：
文献类型：
--
作者：
Morris R;Fagan-Murphy A;MacEachern SJ;Covill D;Patel BA
各种研究都集中在了解粘膜5-羟色胺(5-HT)与结肠动力之间的关系，然而，相互矛盾的研究质疑了这一肠道递质的重要性。在这里，我们描述了一种可以同时检测肠腔内5-羟色胺释放和结肠运动的粪便小球电化学传感器的制作和使用。为了产生形状和大小模拟天然粪便颗粒的装置，使用碳纳米管复合电极制造了粪便颗粒传感器装置，这些电极被安装在3D打印组件中。在小球的不同区域包含电极的情况下制造了器件。结果表明，该装置对5-羟色胺的体外检测是稳定和灵敏的，与天然粪便颗粒相比，粪便颗粒的速度没有差异。在大便小球移动之前观察到粘膜5-羟色胺的开始。粘膜5-羟色胺的释放发生在口服粪便小球时，并与驱动小球推进的肠壁收缩有关。综上所述，这些发现为粘膜5-羟色胺的作用提供了新的见解，并表明该递质是粪便颗粒推进的关键启动者。
Various investigations have focused on understanding the relationship between mucosal serotonin (5-HT) and colonic motility, however contradictory studies have questioned the importance of this intestinal transmitter. Here we described the fabrication and use of a fecal pellet electrochemical sensor that can be used to simultaneously detect the release of luminal 5-HT and colonic motility. Fecal pellet sensor devices were fabricated using carbon nanotube composite electrodes that were housed in 3D printed components in order to generate a device that had shape and size that mimicked a natural fecal pellet. Devices were fabricated where varying regions of the pellet contained the electrode. Devices showed that they were stable and sensitive for ex vivo detection of 5-HT, and no differences in the fecal pellet velocity was observed when compared to natural fecal pellets. The onset of mucosal 5-HT was observed prior to the movement of the fecal pellet. The release of mucosal 5-HT occurred oral to the fecal pellet and was linked to the contraction of the bowel wall that drove pellet propulsion. Taken, together these findings provide new insights into the role of mucosal 5-HT and suggest that the transmitter acts as a key initiator of fecal pellet propulsion.