Detection of human papillomavirus in squamous cell carcinomas of the lung by polymerase chain reaction
Detection of human papillomavirus in squamous cell carcinomas of the lung by polymerase chain reaction
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DOI：
10.1165/ajrcmb.18.2.3033
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发表时间：
1998-02-01
影响因子：
6.4
通讯作者：
Shroyer, KR
中科院分区：
文献类型：
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作者：
Bohlmeyer, T;Le, TN;Shroyer, KR
现有证据支持人乳头瘤病毒（HPV）可能在鳞状上皮细胞恶性转化中发挥病因作用的假设。尽管在子宫颈、肛门直肠、皮肤和上呼吸道的鳞状细胞癌 (SCC) 中检测到 HPV DNA 的比例很高，但很少有研究在肺部 SCC 中检测 HPV。为了确认肺鳞状细胞癌中是否存在 HPV，我们通过聚合酶链反应 (PCR) 检测了从 34 名患者的福尔马林固定组织中提取的 HPV DNA。使用 HPV L1 共有序列引物（MY11 和 MY09；Perkin-Elmer Cetus，Norwalk，CT）进行 DNA 扩增，该引物可识别广泛的 HPV 类型，包括 6、11、16、18、31 和 33 等许多其他已知类型，以及至少 20 种其他未识别类型。 PCR 产物通过琼脂糖凝胶电泳和 Southern blot 杂交与 [P-32] 标记的通用 HPV 探针进行分析。随后，对 PCR 产物与 HPV 6、11、16、18 和 33 型特异性探针进行狭缝印迹杂交，对 HPV DNA 阳性病例进行分析。在 2 例病例中检测到 HPV 18 型，其中 1 例来自 44 岁女性，1 例来自 64 岁男性，其余 32 例呈阴性。在所有病例中，HPV DNA 原位杂交均未能检测到 HPV 6/11、16/18 或 31/33/35 型。我们的结论是，一小部分原发性肺鳞状细胞癌病例的 HPV 18 型检测呈阳性，但绝大多数病例与 HPV 无关。
Existing evidence supports the hypothesis that human papillomavirus (HPV) may play an etiologic role in the malignant transformation of squamous epithelial cells. Although HPV DNA has been identified in a high proportion of squamous cell carcinomas (SCC) of the cervix, anorectum, skin, and upper airways, few studies have tested for HPV in SCC of the lung. To confirm the presence of HPV in lung SCC, we tested for HPV DNA extracted from formalin-fixed tissues of 34 patients by polymerase chain reaction (PCR). DNA amplification was performed using HPV L1 consensus sequence primers (MY11 and MY09; Perkin-Elmer Cetus, Norwalk, CT) which recognize a broad spectrum of HPV types including 6, 11, 16, 18, 31, and 33, among many other known types, as well as at least 20 other unidentified types. PCR products were analyzed by agarose gel electrophoresis and Southern blot hybridization with [P-32]-labeled generic HPV probes. HPV DNA positive cases were subsequently analyzed by slot-blot hybridization of the PCR products with specific probes for HPV types 6, 11, 16, 18, and 33. HPV type 18 was detected in two cases, including one case from a 44-year-old female and one from a 64-year-old male, with the remaining 32 cases negative. in situ hybridization for HPV DNA failed to detect HPV types 6/11, 16/18, or 31/33/35 in any of the cases. We conclude that a small proportion of cases of primary pulmonary SCC test positive for HPV type 18 but that the great majority of cases are not associated with HPV.