Paecilomyces variotii extracts (ZNC) enhance plant immunity and promote plant growth
Paecilomyces variotii extracts (ZNC) enhance plant immunity and promote plant growth
复制标题
拟青霉提取物（ZNC）增强植物免疫力，促进植物生长
DOI：
10.1007/s11104-019-04130-w
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发表时间：
2019-08-01
期刊：
影响因子：
4.9
通讯作者：
Ding, Xinhua
中科院分区：
文献类型：
--
作者：
Lu, Chongchong;Liu, Haifeng;Ding, Xinhua
背景与目的植物内生真菌多变拟青霉（Paecilomycesvariotii，ZNC）的粗提物具有促进植物生长和增强抗病性的作用，在我国被广泛应用。我们的研究旨在评估植物生长促进和疾病保护的分子机制。方法我们使用RNA测序从ZNC处理的幼苗中产生转录组谱。水杨酸（SA）在ZNC介导的免疫中的功能使用SA生物合成和信号通路突变体进行了检查。测定了ZNC处理下幼苗氮、磷含量。使用含有DR 5：：GFP的转基因植物测试ZNC对根中激素生长素水平的影响。结果ZNC在1-10 ng/ml浓度范围内均表现出明显的促生和抗病活性。ZNC诱导ROS积累、胼胝质沉积和PR基因表达。SA的生物合成和信号转导途径所需的ZNC介导的防御反应。结论ZNC是一种高效的植物诱导剂，在低浓度下通过诱导根尖生长素积累促进植物生长，在高浓度下通过激活SA信号通路增强植物抗病性。
Background and aims The crude extract of the endophyte Paecilomyces variotii known as ZhiNengCong (ZNC) has function of promoting plant growth and enhancing disease resistance and is widely used in China. Our study aims to evaluate the molecular mechanisms of plant growth promotion and disease protection. Methods We generated transcriptome profiles from ZNC-treated seedlings using RNA sequencing. The function of salicylic acid (SA) in ZNC-mediated immunity was examined using SA biosynthesis and signaling pathway mutants. The concentrations of nitrogen (N) and phosphorus (P) in seedlings under ZNC treatment were measured. The effect of ZNC on the level of the hormone auxin in roots was tested using transgenic plants containing DR5::GFP. Results ZNC exhibited ultrahigh activity in promoting plant growth and enhancing disease resistance, even at concentrations as low as 1-10 ng/ml. ZNC induced ROS accumulation, callose deposition, and expression of PR genes. SA biosynthesis and signaling pathways were required for the ZNC-mediated defense response. Moreover, in improving plant growth, ZNC increased the level of auxin in root tips and regulated the absorption of N and P. Conclusion According to these results, ZNC is a highly effective plant elicitor that promotes plant growth by inducing auxin accumulation at the root tip at low concentrations and enhances plant disease resistance by activating the SA signaling pathway at high concentrations.