(-)-epicatechin gallate accumulates in foamy macrophages in human atherosclerotic aorta: Implication in the anti-atherosclerotic actions of tea catechins
(-)-epicatechin gallate accumulates in foamy macrophages in human atherosclerotic aorta: Implication in the anti-atherosclerotic actions of tea catechins
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DOI：
10.1016/j.bbrc.2008.07.086
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发表时间：
2008-09-26
影响因子：
3.1
通讯作者：
Terao, Junji
中科院分区：
文献类型：
--
作者：
Kawai, Yoshichika;Tanaka, Hiroko;Terao, Junji
茶儿茶素的生物活性包括抗动脉粥样硬化活性的定位和靶位点尚未完全了解。为了鉴定儿茶素在体内的靶位点，我们已经开发了一种新的单克隆抗体（mAb 5A 3）特异性的（-）-表儿茶素-3-没食子酸酯（ECg），一种主要的茶儿茶素。在人动脉粥样硬化病变中检测到mAb 5A 3的免疫反应物质，而在正常主动脉中未检测到，并且特异性地定位于巨噬细胞源性泡沫细胞。使用巨噬细胞样细胞系的合适的体外实验也显示ECg在细胞中的显著积累。我们还证明，心电图可以抑制清道夫受体CD 36，泡沫细胞形成的关键分子，在巨噬细胞的基因表达。这些结果，第一次，显示了目标网站的茶成分心电图在主动脉，并可能提供了一个机制的儿茶素抗动脉粥样硬化的行动。(C)2008年爱思唯尔公司All rights reserved.
The localization and target sites of tea catechins underlying their biological activity including anti-atherosclerotic activity have not yet been fully understood. To identify the target sites of catechins in vivo, we have developed a novel monoclonal antibody (mAb5A3) specific for (-)-epicatechin-3-gallate (ECg), one of the major tea catechins. The immunoreactive materials with mAb5A3 were detected in the human atherosclerotic lesions but not in the normal aorta, and were specifically localized in the macrophage-derived foam cells. fit vitro experiments using macrophage-like cell lines also showed the significant accumulation of ECg in the cells. We also demonstrated that ECg could suppress the gene expression of a scavenger receptor CD36, a key molecule for foam cell formation, in macrophage cells. These results, for the first time, showed the target site of a tea component ECg in the aorta and might provide a mechanism for the anti-atherosclerotic actions of the catechins. (C) 2008 Elsevier Inc. All rights reserved.