Single administration of di(n-butyl) phthalate delays spermatogenesis in prepubertal rats
Single administration of di(n-butyl) phthalate delays spermatogenesis in prepubertal rats
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DOI：
10.1016/j.tice.2010.02.004
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发表时间：
2010-04-01
期刊：
影响因子：
2.6
通讯作者：
Kurohmaru, Masamichi
中科院分区：
文献类型：
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作者：
Alam, Mohammad Shah;Andrina, Bibin Bintang;Kurohmaru, Masamichi
在第一次精子发生过程中，对 3 周龄大鼠单次施用邻苯二甲酸二丁酯 (DBP) 引起的生精小管形态学变化进行了研究。单次给予DBP（500 mg/kg），治疗后3小时显示生精细胞进行性脱离和移位，肾小管管腔消失，然后在第1天（D1）显示薄的生精上皮和宽的肾小管管腔。 D1时检测到大量凋亡的生精细胞，随后随时间的推移逐渐减少。相比之下，与对照组相比，DBP 治疗组即使在第一波精子发生完成后，睾丸的体重增加也较低。为了阐明DBP处理的大鼠中生精细胞是否分化为成熟精子细胞，对细长精子细胞的特异性标记物Hsc 70t进行了免疫组织化学染色。结果表明，与对照组相比，DBP 处理的睾丸中成熟精子细胞减少。例如，在治疗后的D20（41日龄），DBP治疗组大鼠肾小管中最先进的精子细胞为步骤2-4，而对照组的精子细胞为步骤12-13。此外，在一些小管中，粗线期精母细胞是最先进的生精细胞。治疗后第30天（51日龄），DBP治疗组大鼠生精细胞成熟进一步进行，小管中最先进的精子细胞为第8-9步，而对照组为第15-19步。这些结果使我们得出这样的假设：即使在第一波精子发生完成后，对青春期前的大鼠单次施用 DBP 也会延迟生精细胞的成熟。 (C) 2010 Elsevier Ltd. 保留所有权利。
Morphological alterations in seminiferous tubules caused by single administration of di(n-butyl) phthalate (DBP) in 3-week-old rats were investigated throughout the first wave of spermatogenesis. Single administration of DBP (500 mg/kg) showed progressive detachment and displacement of spermatogenic cells and disappearance of tubular lumen at 3 h after treatment, and then showed thin seminiferous epithelia and wide tubular lumen at day 1 (D1). At D1, quite significant numbers of apoptotic spermatogenic cells were detected, and then they gradually decreased in accordance with the passage of time. In contrast, the testes revealed lower weight gain, even after completion of first wave of spermatogenesis in the DBP-treated group, compared to the control. In order to clarify whether spermatogenic cells differentiate into mature spermatids in the DBP-treated rats, immunohistochemical staining for Hsc 70t, a specific marker for elongate spermatids, was carried out. As a result, the decrease in mature spermatids in the DBP-treated testes, compared to the control, was demonstrated. For example, at D20 (41-day-old) after treatment, the most advanced spermatids in the tubules from rats in the DBP-treated groups were steps 2-4, while those of the control were steps 12-13. Moreover, in some tubules, pachytene spermatocytes were the most advanced spermatogenic cell. At D30 (51-day-old) after treatment, maturation of spermatogenic cells in the DBP-treated rats proceeded further, and the most advanced spermatids in tubules were steps 8-9, while those of the control were steps 15-19. These results lead us to the postulation that a single administration of DBP to prepubertal rats delays maturation of spermatogenic cells, even after completion of first wave of spermatogenesis. (C) 2010 Elsevier Ltd. All rights reserved.