Individual and stable autoantibody repertoires in healthy individuals
Individual and stable autoantibody repertoires in healthy individuals
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DOI：
10.1080/08916934.2019.1581774
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发表时间：
2019-01-02
期刊：
影响因子：
3.5
通讯作者：
Nilsson, Peter
中科院分区：
文献类型：
--
作者：
Neiman, Maja;Hellstrom, Cecilia;Nilsson, Peter
在迈向精准医学的时代，我们在这里展示了193名健康个体的个体特异性自身抗体特征。自身反应性IgG特征随时间推移是稳定的，其方式是在纵向采样中识别每个个体谱。在来自193名健康个体的纵向血浆样品中测量了IgG自身抗体对包含335个蛋白质片段的抗原阵列的反应性，所述蛋白质片段代表与自身免疫性疾病潜在相关的204种人蛋白质。该分析产生了每个个体的独特自身抗体条形码，其保持了一年的时间。反应性特征或特征在反应性数量和抗原特异性方面具有个体特异性。两个独立的数据集是一致的，因为每个健康个体显示出对0-16种抗原的反应性，中位数为6种。随后，在内部生产的高密度蛋白质阵列上对四个选定的个体进行了分析，该阵列包含代表14，000个独特蛋白质编码基因的23，000个蛋白质片段。基于自身抗体反应性的独特，广泛和深入的纵向分析，我们的结果表明，在每个分析的健康个体中具有独特的自身反应性特征。对健康个体的宽范围和高分辨率分子谱分析的需求和兴趣正在上升。我们在这里产生和评估的自我反应性IgG库在健康个体的全球分布的初步观点，通过调查193个良好的特征健康个体。
In the era towards precision medicine, we here present the individual specific autoantibody signatures of 193 healthy individuals. The self-reactive IgG signatures are stable over time in a way that each individual profile is recognized in longitudinal sampling. The IgG autoantibody reactivity towards an antigen array comprising 335 protein fragments, representing 204 human proteins with potential relevance to autoimmune disorders, was measured in longitudinal plasma samples from 193 healthy individuals. This analysis resulted in unique autoantibody barcodes for each individual that were maintained over one year's time. The reactivity profiles, or signatures, are person specific in regards to the number of reactivities and antigen specificity. Two independent data sets were consistent in that each healthy individual displayed reactivity towards 0-16 antigens, with a median of six. Subsequently, four selected individuals were profiled on in-house produced high-density protein arrays containing 23,000 protein fragments representing 14,000 unique protein coding genes. Based on a unique, broad and deep longitudinal profiling of autoantibody reactivities, our results demonstrate a unique autoreactive profile in each analyzed healthy individual. The need and interest for broad-ranged and high-resolution molecular profiling of healthy individuals is rising. We have here generated and assessed an initial perspective on the global distribution of the self-reactive IgG repertoire in healthy individuals, by investigating 193 well-characterized healthy individuals.