Characterization of plant miRNAs and small RNAs derived from potato spindle tuber viroid (PSTVd) in infected tomato
Characterization of plant miRNAs and small RNAs derived from potato spindle tuber viroid (PSTVd) in infected tomato
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DOI：
10.1515/bc.2010.148
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发表时间：
2010-12-01
影响因子：
3.7
通讯作者：
Steger, Gerhard
中科院分区：
文献类型：
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作者：
Diermann, Natalie;Matousek, Jaroslav;Steger, Gerhard
为了防御入侵的病原体，植物具有涉及小RNA（miRNAs，siRNAs）的RNA沉默机制。此外，类病毒-植物感染性、非编码、未标记的RNA -引起类病毒特异性小RNA（vsRNA）的产生，但类病毒确实逃脱了细胞质沉默机制。具有微小序列变异的类病毒可以在受感染的植物中产生不同的症状，这表明vsRNA参与了症状的产生。我们通过深度测序分析了PSTVd菌株AS 1诱导的vsRNA谱，该菌株AS 1在感染番茄植物品种Rutgers后引起强烈症状，如矮化和坏死。事实上，发现频率最高的vsRNA映射到PSTVd的致病性调节结构域，支持vsRNA参与症状的产生。此外，在PSTVd AS 1感染的植物中，通过调节转录因子参与叶发育的一些内源性miRNA的积累受到抑制。后一项发现支持了转录因子的miRNA依赖性（错误）调节导致类病毒症状的假设。
To defend against invading pathogens, plants possess RNA silencing mechanisms involving small RNAs (miRNAs, siRNAs). Also viroids - plant infectious, non-coding, unencapsidated RNA - cause the production of viroid-specific small RNAs (vsRNA), but viroids do escape the cytoplasmic silencing mechanism. Viroids with minor sequence variations can produce different symptoms in infected plants, suggesting an involvement of vsRNAs in symptom production. We analyzed by deep sequencing the spectrum of vsRNAs induced by the PSTVd strain AS1, which causes strong symptoms such as dwarfing and necrosis upon infection of tomato plants cv Rutgers. Indeed, vsRNAs found with highest frequency mapped to the pathogenicity-modulating domain of PSTVd, supporting an involvement of vsRNAs in symptom production. Furthermore, in PSTVd AS1-infected plants the accumulation of some endogenous miRNAs, which are involved in leaf development via regulation of transcription factors, is suppressed. The latter finding supports the hypothesis that a miRNA-dependent (mis) regulation of transcription factors causes the viroid symptoms.