Femtosecond diffractive imaging of biological cells
Femtosecond diffractive imaging of biological cells
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DOI：
10.1088/0953-4075/43/19/194015
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发表时间：
2010-10-14
影响因子：
1.6
通讯作者：
Hajdu, Janos
中科院分区：
文献类型：
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作者：
Seibert, M. Marvin;Boutet, Sebastien;Hajdu, Janos
在闪光衍射实验中，一个短而极强的X射线脉冲照射样品，以获得显着的辐射损伤开始之前的衍射图案。过采样衍射图案允许通过迭代定相方法进行相位恢复。闪光衍射成像首先在无机测试对象上被证明（Chapman等人，2006 Nat.Phys.2 839-43）。我们在这里报告的生物系统中，个别细胞进行成像的实验，使用单一的，10-15 fs的软X射线脉冲在13.5 nm波长的FLASH自由电子激光在汉堡。模拟结果表明，脉冲加热样品到约160 000 K，但没有之前可以获得可解释的衍射图案。重建的投影图像返回完整细胞的结构。模拟表明，在脉冲期间，最热表面层中的离子和原子的平均位移保持在3埃以下。
In a flash diffraction experiment, a short and extremely intense x-ray pulse illuminates the sample to obtain a diffraction pattern before the onset of significant radiation damage. The over-sampled diffraction pattern permits phase retrieval by iterative phasing methods. Flash diffractive imaging was first demonstrated on an inorganic test object (Chapman et al 2006 Nat. Phys. 2 839-43). We report here experiments on biological systems where individual cells were imaged, using single, 10-15 fs soft x-ray pulses at 13.5 nm wavelength from the FLASH free-electron laser in Hamburg. Simulations show that the pulse heated the sample to about 160 000 K but not before an interpretable diffraction pattern could be obtained. The reconstructed projection images return the structures of the intact cells. The simulations suggest that the average displacement of ions and atoms in the hottest surface layers remained below 3 angstrom during the pulse.