A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-D-glucan
A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-D-glucan
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DOI：
10.1016/j.carbpol.2014.06.072
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发表时间：
2014-11-26
影响因子：
11.2
通讯作者：
Lopez, Paloma
中科院分区：
文献类型：
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作者：
Laura Werning, Maria;Perez-Ramos, Adrian;Lopez, Paloma
乳酸菌合成的胞外多糖具有益生元特性，有助于发酵食品的流变学和质地。在这里，我们标准化了一种特异性检测2-取代(1,3)- β - d -葡聚糖的免疫学方法。该方法允许在含有其他单糖和多糖的培养上清液中直接检测和定量该外多糖。此外，它还可以对体外酶促反应中合成的生物分子进行特异性检测。因此，该方法可以快速鉴定产生细菌，以及负责其合成的糖基转移酶的生化表征。(C) 2014 Elsevier Ltd.版权所有。
Exopolysaccharides synthesized by lactic acid bacteria have prebiotic properties and contribute to the rheology and texture of fermented foods. Here, we have standardized an immunological method for the specific detection of 2-substituted (1,3)-beta-D-glucans. The method allows direct detection and quantification of this exopolysaccharide in culture supernatants containing other mono- and poly-saccharides. Moreover, it allows specific detection of the biomolecules synthesized in vitro in enzymatic reactions. Thus, this method allows the fast identification of producing bacteria, as well as biochemical characterization of the glycosyltransferases responsible for their synthesis. (C) 2014 Elsevier Ltd. All rights reserved.