Decreased expression of specific genes in yeast cells lacking histone H1
Decreased expression of specific genes in yeast cells lacking histone H1
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DOI：
10.1074/jbc.m011196200
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发表时间：
2001-04-27
影响因子：
4.8
通讯作者：
Turcotte, B
中科院分区：
文献类型：
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作者：
Hellauer, K;Sirard, E;Turcotte, B
染色质在调节真核基因表达中发挥重要作用。染色质由包裹在核小体核心（由两个高度保守的组蛋白 H2A、H2B、H3 和 H4 副本组成）和一个更加可变的连接组蛋白 H1 的 DNA 组成。各种体外和体内研究表明组蛋白 H1 作为基因表达的阻遏物或激活剂，但其确切作用仍不清楚。酵母基因组测序鉴定出了假定的组蛋白 H1 基因。生化研究表明，酵母确实拥有真正的组蛋白 H1。然而，酵母独特的H1基因的缺失与任何表型无关，人们质疑它是否发挥任何作用。为了解决这个问题，我们进行了全基因组微阵列分析，以确定受 H1 去除影响的基因。令人惊讶的是，编码组蛋白 H1 的基因的删除并没有导致基因表达增加，而是导致基因表达适度减少。所选基因的 Northern 印迹分析证实了通过微阵列分析获得的结果。使用集成的 lacZ 报告基因观察到类似的效果。因此，我们的数据表明，去除酵母组蛋白 H1 只会导致基因表达降低。
Chromatin plays an important role in regulating eukaryotic gene expression. Chromatin is composed of DNA wrapped around a nucleosome core (consisting of two copies of the well conserved histones H2A, H2B, H3, and H4) and a more variable linker histone H1. Various in vitro and in vivo studies have implicated histone H1 as a repressor of gene expression or as an activator, but its exact role is still unclear. Sequencing of the yeast genome has led to the identification of a putative histone H1 gene. Biochemical studies demonstrated that yeast does indeed possess a bona fide histone H1. However, deletion of the unique yeast H1 gene is not associated with any phenotypes, and it was questioned whether it plays any role. To address this issue, we performed whole-genome microarray analysis to identify genes that are affected by H1 removal, Surprisingly, deletion of the gene encoding histone H1 does not result in increased gene expression but rather in a modest reduction, Northern blot analysis of selected genes confirmed the results obtained with the microarray analysis. A similar effect was observed with an integrated lacZ reporter. Thus, our data demonstrate that removal of yeast histone H1 only results in decreased gene expression.