High Throughput Synthesis of Uniform Biocompatible Polymer Beads with High Quantum Dot Loading Using Microfluidic Jet-Mode Breakup
High Throughput Synthesis of Uniform Biocompatible Polymer Beads with High Quantum Dot Loading Using Microfluidic Jet-Mode Breakup
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DOI：
10.1021/la4041198
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发表时间：
2014-03-04
期刊：
影响因子：
3.9
通讯作者：
Jensen, Klavs F.
中科院分区：
文献类型：
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作者：
Lee, Seung-Kon;Baek, Jinyoung;Jensen, Klavs F.
采用高通量相干射流分解生物相容性聚乙二醇二丙烯酸酯（PEGDA）预聚树脂，然后进行在线光聚合，制备了具有高负载量子点（QDs）的均匀聚合物微珠。螺旋状和逐渐扩大的通道可以最大限度地吸收辐射的紫外光，从而实现在线光聚合，而不会发生聚结和堵塞问题。虽然滴灌模式通常比喷射模式具有更好的均匀性，但我们的喷嘴设计具有锥形几何形状，可以控制射流破裂，导致3%的均匀粒径分布，与滴灌模式的性能相当。在相同的聚合物流速下，我们实现了2.32 kHz的最大生产速率，比滴注模式快38倍。此外，喷射模式方案提供了更好的通用性，尺寸控制范围扩大了3倍，并且与可能导致微系统压力积聚的粘性流体兼容。作为演示，引入了一种掺杂QD的预聚树脂，以创建均匀的生物相容性聚合物珠，其中CdSe/ZnSe QD负载为10 wt %。尽管负载如此之高，所得到的聚合物珠具有28nm的窄带宽，可用于超灵敏的生物成像、光学编码和单珠传感。
Uniform polymer microbeads with highly loaded quantum dots (QDs) are produced using high-throughput coherent jet breakup of a biocompatible poly(ethylene glycol) diacrylate (PEGDA) prepolymer resin, followed by in-line photopolymerization. A spiraling and gradually widening channel enables maximum absorption of radiated UV light for the in-line photopolymerization without coalescence and clogging issues. Although the dripping mode in general provides superior uniformity to the jet mode, our nozzle design with tapered geometry brings controlled jet breakup leading to 3% of uniform particle size distribution, comparable to dripping-mode performance. We achieve a maximum production rate of 2.32 kHz, 38 times faster than the dripping mode, at a same polymer flow rate. In addition, the jet-mode scheme provides better versatility with 3 times wider range of size control as well as the compatibility with viscous fluids that could cause pressure buildup in the microsystem. As a demonstration, a QD-doped prepolymer resin is introduced to create uniform biocompatible polymer beads with 10 wt % CdSe/ZnSe QD loading. In spite of this high loading, the resulting polymer beads exhibits narrow bandwidth of 28 nm to be used for the ultrasensitive bioimaging, optical coding, and sensing sufficiently with single bead.