OsATM Safeguards Accurate Repair of Meiotic Double-Strand Breaks in Rice(1)
OsATM Safeguards Accurate Repair of Meiotic Double-Strand Breaks in Rice(1)
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OsATM 保障水稻减数分裂双链断裂的精确修复(1)
DOI：
10.1104/pp.20.00053
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发表时间：
2020
期刊：
影响因子：
7.4
通讯作者：
Yu Hengxiu
中科院分区：
文献类型：
--
作者：
Zhang Chao;Zhang Fanfan;Cheng Xinjie;Liu Kangwei;Tang Jiaqi;Li Yafei;Tang Ding;Cheng Zhukuan;Yu Hengxiu
共济失调血管扩张突变（ATM）蛋白在DNA损伤反应中的作用已得到充分研究。然而，它在减数分裂中的作用尚未得到充分研究。在这里，我们的特点是功能的水稻（水稻）ATM同源物在减数分裂。在花粉母细胞（PMCs）同源配对和联会完成后，观察到异常的染色体联合和DNA片段化。Osspo 11 -1 Osatm-1双突变体中染色体异常联合消失，Osdmc 1 Osatm中染色体异常更为严重，表明OsATM在OsSPO 11 -1催化的双链断裂形成下游发挥作用，并与OsDMC 1介导的同源重组平行。我们进一步证明，与体细胞中的情况相反，PMC中H2 AX的磷酸化不依赖于OsATM。此外，OsDMC 1从OsatmPMCs染色体上的去除被延迟，HEI 10灶（干扰敏感的交叉中间体的标记物）的数量减少。总之，这些发现表明OsATM在水稻减数分裂双链断裂的精确修复中起着重要作用。
ATAXIA TELANGIECTASIA-MUTATED (ATM) protein has been well studied for its roles in the DNA damage response. However, its role in meiosis has not been fully explored. Here, we characterized the functions of the rice (Oryza sativa) ATM homolog during meiosis. Aberrant chromosome associations and DNA fragmentations were observed after the completion of homologous pairing and synapsis inOsatmpollen mother cells (PMCs). Aberrant chromosome associations disappeared inOsspo11-1 Osatm-1double mutants and more severe defects were observed inOsdmc1 Osatm, suggesting that OsATM functions downstream of OsSPO11-1-catalyzed double-strand break formation and in parallel with OsDMC1-mediated homologous recombination. We further demonstrated that phosphorylation of H2AX in PMCs did not depend on OsATM, in contrast to the situation in somatic cells. Moreover, the removal of OsDMC1 from chromosomes inOsatmPMCs was delayed and the number of HEI10 foci (markers of interference-sensitive crossover intermediates) decreased. Together, these findings suggest that OsATM plays important roles in the accurate repair of meiotic double-strand breaks in rice.