Immune and acute phase response in pigs experimentally infected with H1N2 swine influenza virus
Immune and acute phase response in pigs experimentally infected with H1N2 swine influenza virus
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DOI：
10.1111/j.1574-695x.2012.01026.x
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发表时间：
2012-12-01
影响因子：
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通讯作者：
Kwit, Krzysztof
中科院分区：
文献类型：
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作者：
Pomorska-Mol, Malgorzata;Markowska-Daniel, Iwona;Kwit, Krzysztof
研究了猪感染h1n1猪流感病毒（SwH1N2）后急性期蛋白（APPs）及其免疫反应。8头仔猪鼻内感染SwH1N2。其中4人作为对照组。采用血凝抑制试验检测猪流感病毒（SIV）的抗体水平。增殖试验用于测量流感特异性细胞介导的反应。在血液学分析仪上测量血液学参数。采用elisa法测定血清中c反应蛋白（CRP）、接触珠蛋白（Hp）、血清淀粉样蛋白A （SAA）和猪主要APP （pig - map）的浓度。从7 dpi开始，在感染猪血清中检测到SwH1N2抗体。从5dpi开始观察swh1n2特异性t细胞反应。在3至7 dpi的所有感染猪中，观察到淋巴细胞数量显著下降，中等细胞（MID）计数增加，未伴有白细胞减少。在感染猪中，当病毒脱落量最大时（从1 dpi到3 dpi）， CRP、Hp和SAA的浓度显著升高。在研究期间，猪- map水平保持不变。血清中SAA的最大浓度与肺评分之间存在显著的正相关，这使得SAA在实验性感染研究（如疫苗效率调查）或疾病严重程度的标记中可能成为有用的指标，但为了证实这一假设，还需要更多的研究。
Acute phase proteins (APPs) and immune responses in pigs after experimental infection with H1N2 swine influenza virus (SwH1N2) were studied. Eight piglets were infected intranasally with SwH1N2. Four served as controls. Antibodies against swine influenza virus (SIV)s were measured by hemagglutination inhibition assay. The proliferation assay was used to measure influenza-specific cell-mediated response. Hematological parameters were measured on a hematology analyzer. C-reactive protein (CRP), haptoglobin (Hp), serum amyloid A (SAA) and pig major APP (Pig-MAP) concentrations in serum were measured using commercial ELISAs. Antibodies against SwH1N2 in the serum of infected pigs were detected from 7 dpi. SwH1N2-specific T-cell response was observed from 5 dpi. A significant drop in lymphocyte numbers and an increase in medium-sized cell (MID) counts with no accompanying leukopenia was observed in all infected pigs from 3 to 7 dpi. In infected pigs, concentrations of CRP, Hp and SAA increased significantly when the greatest amounts of virus were shed (from 1 to 3 dpi). The level of Pig-MAP remained unchanged during study. The significant positive correlation found between maximum concentrations of SAA in serum and lung scores, makes SAA a potentially useful indicator in experimental infection studies (e.g. vaccine efficiency investigations) or as a marker for disease severity, but to confirm this hypothesis more studies are needed.