The human and mouse Period1 genes:: Five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription
The human and mouse Period1 genes:: Five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription
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DOI：
10.1006/geno.2000.6166
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发表时间：
2000-05-01
期刊：
影响因子：
4.4
通讯作者：
Tei, H
中科院分区：
文献类型：
--
作者：
Hida, A;Koike, N;Tei, H
对人和小鼠的时钟基因Perod1进行了序列测定和特征分析。人PERIOD1(人PER1)和小鼠PER1(小鼠PER1)均由23个外显子组成，大小约为16kb，其结构具有很强的相似性。例如，在5‘上游序列中发现了6个高度保守的区域。这些保守片段具有77-88%的同源性，具有几个潜在的调控元件，包括5个E-box(Clock-BMAL1复合体的结合部位)和4个环状AMP反应元件。利用mPer1-荧光素酶融合基因的瞬时转染分析表明，每个保守的E-box都可以作为mCLOCK和mBMAL1反式激活mPer1的增强子。(C)2000年学术出版社。
The clock gene, Period1, from human and mouse was sequenced and characterized. Both human PERIOD1 (human PER1) and mouse Period1 (mouse Per1) consisted of 23 exons spanning approximately 16 kb, and their structures showed strong similarity to each other. For example, six highly conserved regions were identified in the 5' upstream sequences. These conserved segments exhibited 77-88% identity and possessed several potential regulatory elements including five E-boxes (the binding site of the CLOCK-BMAL1 complex) and four cyclic AMP response elements. Transient transfection assays using a mPer1-luciferase fusion gene revealed that each of the conserved E-boxes additively functions as an enhancer for the transactivation of mPer1 by mCLOCK and mBMAL1. (C) 2000 Academic Press.