Regulation of polar peptidoglycan biosynthesis by Wag31 phosphorylation in mycobacteria.
Regulation of polar peptidoglycan biosynthesis by Wag31 phosphorylation in mycobacteria.
复制标题
DOI：
10.1186/1471-2180-10-327
复制
发表时间：
2010-12-29
期刊：
影响因子：
4.2
通讯作者：
Kang CM
中科院分区：
文献类型：
--
作者：
Jani C;Eoh H;Lee JJ;Hamasha K;Sahana MB;Han JS;Nyayapathy S;Lee JY;Suh JW;Lee SH;Rehse SJ;Crick DC;Kang CM
对环境变化的感知和反应是细胞分裂调节的一个核心方面。结核分枝杆菌含有11种Ser/Thr激酶，其中两种PknA和PknB是调节细胞分裂/形态的关键信号分子。这些激酶的底物之一是Wag 31，我们以前表明，Wag 31的部分耗尽引起的形态学变化，指示细胞壁缺陷，并且Wag 31的磷酸化状态影响分枝杆菌中的细胞生长。在本研究中，我们进一步表征了Wag 31磷酸化在极性肽聚糖生物合成中的作用。我们表明，表达不同的wag 31等位基因（野生型，磷酸消融，或磷酸模拟）的细胞之间的差异生长是由不同的新生肽聚糖的生物合成，至少部分。磷酸化状态的Wag 31被发现是重要的Wag 31分子之间的蛋白质-蛋白质相互作用，因此，其极性定位。与这些结果一致，表达拟磷酸化wag 31等位基因的细胞在肽聚糖生物合成途径中具有更高的酶活性。Wag 31 Mtb磷酸化是Wag 31 Mtb调节肽聚糖合成从而调节分枝杆菌最佳生长的一种新的分子机制。
Sensing and responding to environmental changes is a central aspect of cell division regulation. Mycobacterium tuberculosis contains eleven Ser/Thr kinases, two of which, PknA and PknB, are key signaling molecules that regulate cell division/morphology. One substrate of these kinases is Wag31, and we previously showed that partial depletion of Wag31 caused morphological changes indicative of cell wall defects, and that the phosphorylation state of Wag31 affected cell growth in mycobacteria. In the present study, we further characterized the role of the Wag31 phosphorylation in polar peptidoglycan biosynthesis. We demonstrate that the differential growth among cells expressing different wag31 alleles (wild-type, phosphoablative, or phosphomimetic) is caused by, at least in part, dissimilar nascent peptidoglycan biosynthesis. The phosphorylation state of Wag31 is found to be important for protein-protein interactions between the Wag31 molecules, and thus, for its polar localization. Consistent with these results, cells expressing a phosphomimetic wag31 allele have a higher enzymatic activity in the peptidoglycan biosynthetic pathway. The Wag31Mtb phosphorylation is a novel molecular mechanism by which Wag31Mtb regulates peptidoglycan synthesis and thus, optimal growth in mycobacteria.
登录
查看更多内容
影响因子：
14.9
作者：
Blokpoel MC;Murphy HN;O'Toole R;Wiles S;Runn ES;Stewart GR;Young DB;Robertson BD
通讯作者：
Robertson BD
影响因子：
10.5
作者：
Kang, CM;Abbott, DW;Husson, RN
通讯作者：
Husson, RN
影响因子：
5.6
作者：
Parikh, Amit;Verma, Sunil Kumar;Nandicoori, Vinay Kumar
通讯作者：
Nandicoori, Vinay Kumar
影响因子：
10.5
作者：
Marston, AL;Thomaides, HB;Errington, J
通讯作者：
Errington, J
影响因子：
4.8
作者：
Fiuza, Maria;Canova, Marc J.;Gil, Jose A.
通讯作者：
Gil, Jose A.