Reduction in peripheral nerve allograft antigenicity with warm and cold temperature preservation.
Reduction in peripheral nerve allograft antigenicity with warm and cold temperature preservation.
复制标题
冷热保存可降低周围神经同种异体移植物的抗原性。
DOI：
10.1097/00006534-199601000-00025
复制
发表时间：
1996
影响因子：
3.6
通讯作者：
Hay,JB
中科院分区：
文献类型：
--
作者：
Strasberg,SR;Mackinnon,SE;Hare,GM;Narini,PP;Hertl,C;Hay,JB
评估淋巴细胞向新鲜和保存的周围神经移植物中的迁移，以确定保存时间、保存温度和移植物收获技术对周围神经移植物免疫反应的影响。腓神经取材于活体供体或尸体供体（组织），制成1.5 cm的神经段，在5℃或37℃下保存1、3、5或7天。9只近交系母羊分别接受多段自体腓骨移植物、新鲜同种异体移植物和保存的同种异体神经移植物。通过静脉注射自体111in标记淋巴细胞，观察植入7天后淋巴细胞的迁移情况，并用伽马计数仪定量。淋巴细胞迁移到新鲜同种异体移植物组织（7212+/-1575）比新鲜自体移植物组织（1758+/-421;p< 0.05）平均增加4.1倍。与新鲜同种异体移植物（7212+/-1575）相比，在这两种温度下短期保存（24小时）均能增强淋巴细胞向预处理同种异体移植物组织（5[度]C时12684+/-2575,37[度]C时8751+/-1577）的迁移。相反，在5[度]C（3586+/-1421）和37[度]C（1570+/-414）条件下进行7天的预处理，其迁移值与自体移植物没有显著差异。低温保存5天后，活体（5710+/-1651）和尸体（组织）供体（4013+/-832）的移植物之间没有统计学上的显著差异。Reconstr。外科杂志97:152,1996。)
: Lymphocyte migration into fresh and preserved peripheral nerve allografts was assessed to determine the effects of preservation lime, preservation temperature, and graft harvest technique on the immunologic response to the peripheral nerve allograft. Peroneal nerve was harvested from either live or cadaveric (tissue) donors and stored as 1.5-cm segments at 5 [degrees] C or 37 [degrees] C for 1, 3, 5, or 7 days. Each of nine outbred ewes then received multiple segments of peroneal autograft, fresh allograft, and preserved nerve allograft implants. Lymphocyte migration was studied 7 days after implantation by intravenous injection of autologous 111In-labeled lymphocytes and quantified by gamma counter. Lymphocyte migration into fresh allografts (7212+/-1575) increased an average of 4.1 times over fresh autograft tissue (1758+/-421; p< 0.05). Short-term preservation (24 hours) at both temperatures enhanced lymphocyte migration into pretreated allograft tissue (12684+/-2575 at 5 [degrees] C, 8751+/-1577 at 37 [degrees] C) as compared with fresh allograft (7212+/-1575). Conversely, 7 days of pretreatment at both 5 [degrees] C (3586+/-1421) and 37 [degrees] C (1570+/-414) resulted in migration values not significantly different from autograft. No statistically significant difference was seen between grafts harvested from live (5710+/-1651) versus cadaveric (tissue) donors (4013+/-832) after 5 days of cold preservation.(Plast. Reconstr. Surg. 97: 152, 1996.)