Anti-Human Rhinovirus Activity of Gallic Acid Possessing Antioxidant Capacity
Anti-Human Rhinovirus Activity of Gallic Acid Possessing Antioxidant Capacity
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DOI：
10.1002/ptr.3101
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发表时间：
2010-09-01
影响因子：
7.2
通讯作者：
Baek, Seung Hwa
中科院分区：
文献类型：
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作者：
Choi, Hwa Jung;Song, Jae Hyoung;Baek, Seung Hwa
人类鼻病毒(HRV)是普通感冒的主要原因，到目前为止，还没有登记的临床有效的抗病毒化疗药物来治疗由HRV引起的疾病。我们以前的报道表明，从五味子花中提取的没食子酸具有抗氧化活性。许多研究报道抗氧化剂对多种病毒具有抗病毒活性。因此，我们通过观察没食子酸对HRV诱导的细胞病变效应(CPE)和HRV颗粒的感染性的影响，检测了没食子酸对HRV的抗病毒活性及其作用方式，并进行了时间添加研究。结果表明，没食子酸对人上皮样癌宫颈癌细胞(HeLa)的HRV2和HRV3复制有明显的抑制作用，其抗病毒活性大于55%，且无细胞毒性。利巴韦林的抗HRV3活性低于没食子酸，而抗HRV3的活性与没食子酸相似。在HRV感染的HeLa细胞中加入没食子酸直接减少了可见的CPE的形成。此外，没食子酸确实与HRV颗粒直接相互作用或激活。总之，我们得出结论，没食子酸抑制HRV的产生主要是由于作为抗氧化剂的一般作用和抑制病毒吸收的作用方式所致。版权所有(C)2010 John Wiley&Sons，Ltd.
Human rhinoviruses (HRVs) are a major cause of the common cold and until now there is no registered clinically effective antiviral chemotherapeutic agent for treatment of diseases caused by HRVs. Our previous report showed that gallic acid from Woodfordia fruticosa flowers possessed antioxidant activity. Many studies reported that antioxidants possess antiviral activities against various viruses. Therefore, we examined antiviral activity of gallic acid against HRVs and mode of its actions by observing the effect of gallic acid on HRV-induced cytopathic effect (CPE) and the infectivity of HRV particles, and then carried out a time-addition study. As a result, gallic acid actively inhibited HRV2 and -3 replications with antiviral activity more than 55% without cytotoxicity in human epitheloid carcinoma cervix (HeLa) cells at a concentration of 100 mu g/mL. Also, ribavirin showed lower anti-HRV3 activity than gallic acid and similar anti-HRV3 activity to it. The addition of gallic acid to HRV-infected HeLa cells directly reduced the formation of a visible CPE. Furthermore, gallic acid did directly interact or activate with HRV particles. Collectively, we concluded that the inhibition of HRV production by gallic acid is mainly due to a general action as an antioxidant and the mode of action derived from the inhibition of virus absorption. Copyright (C) 2010 John Wiley & Sons, Ltd.