Comparative analysis of the cardiac structure and transcriptome of scallop and snail, perspectives on heart chamber evolution
Comparative analysis of the cardiac structure and transcriptome of scallop and snail, perspectives on heart chamber evolution
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DOI：
10.1007/s42995-023-00202-0
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发表时间：
2023-11
影响因子：
5.7
通讯作者：
Meina Lu;Rabia Hayat;Xuejiao Zhang;Yaqi Jiao;Jianyun Huang;Y. Huangfu;Mingcan Jiang;Jieyi Fu;Qingqiu Jiang;Yaojia Gu;Shi Wang;Alexander A. Akerberg;Ying Su;Long Zhao
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作者：
Meina Lu;Rabia Hayat;Xuejiao Zhang;Yaqi Jiao;Jianyun Huang;Y. Huangfu;Mingcan Jiang;Jieyi Fu;Qingqiu Jiang;Yaojia Gu;Shi Wang;Alexander A. Akerberg;Ying Su;Long Zhao
具有心房和心室的双腔心脏的进化改善了后口动物（脊椎动物）和一些原口动物（无脊椎动物）的心脏功能。虽然研究已经检查了这两个腔室的独特结构和功能，但分子比较很少并且仅限于脊椎动物。在这里，我们专注于软体动物的两室原口心脏，提供的数据，可以提供一个更好的了解心脏的进化。具体来说，我们询问了心房和心室在软体动物心脏的分子水平上是否不同。为了做到这一点，我们研究了两个非常不同的物种，巨大的非洲陆地蜗牛（Lissachatina fulica）和相对较小的水生虾夷扇贝（Mizuhopecten yessoensis），假设如果它们表现出共性，这些相似性可能会反映出整个门。我们发现，虽然这两个物种的心脏不同的组织学，他们的心脏基因功能富集是相似的，所揭示的转录组学分析。此外，每个物种的心房和心室有不同的基因功能簇，表明在软体动物的心脏腔室的进化分化。最后，为了探索脊椎动物和无脊椎动物两室心脏之间的关系，我们将我们的转录组数据与斑马鱼的已发表数据进行了比较，斑马鱼是一种研究充分的两室心脏脊椎动物模型。我们的分析表明，软体动物和斑马鱼之间的心室基因的功能相似性，这表明心室的分化，以实现相同的功能，在无脊椎动物和脊椎动物。作为对原口动物的第一项此类研究，我们的研究结果提供了对双腔心脏如何产生的初步见解，包括对原口动物和后口动物中发生的可能理解。
The evolution of a two-chambered heart, with an atrium and a ventricle, has improved heart function in both deuterostomes (vertebrates) and some protostomes (invertebrates). Although studies have examined the unique structure and function of these two chambers, molecular comparisons are few and limited to vertebrates. Here, we focus on the two-chambered protostome heart of the mollusks, offering data that may provide a better understanding of heart evolution. Specifically, we asked if the atrium and ventricle differ at the molecular level in the mollusk heart. To do so, we examined two very different species, the giant African land snail (Lissachatina fulica) and the relatively small, aquatic yesso scallop (Mizuhopecten yessoensis), with the assumption that if they exhibited commonality these similarities would likely reflect those across the phylum. We found that, although the hearts of these two species differed histologically, their cardiac gene function enrichments were similar, as revealed by transcriptomic analysis. Furthermore, the atrium and ventricle in each species had distinct gene function clusters, suggesting an evolutionary differentiation of cardiac chambers in mollusks. Finally, to explore the relationship between vertebrate and invertebrate two-chambered hearts, we compared our transcriptomic data with published data from the zebrafish, a well-studied vertebrate model with a two-chambered heart. Our analysis indicated a functional similarity of ventricular genes between the mollusks and the zebrafish, suggesting that the ventricle was differentiated to achieve the same functions in invertebrates and vertebrates. As the first such study on protostomes, our findings offered initial insights into how the two-chambered heart arose, including a possible understanding of its occurrence in both protostomes and deuterostomes.