HTR-8/SVneo cell line contains a mixed population of cells
HTR-8/SVneo cell line contains a mixed population of cells
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DOI：
10.1016/j.placenta.2016.12.007
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发表时间：
2017-02-01
期刊：
影响因子：
3.8
通讯作者：
Daoud, Georges
中科院分区：
文献类型：
--
作者：
Abou-Kheir, Wassim;Barrak, Joanna;Daoud, Georges
前言：胎盘是人类的暂时性器官，对妊娠维持和胎儿发育至关重要。滋养层细胞和基质细胞是人类胎盘中存在的主要细胞类型。滋养层细胞是滋养外胚层的衍生体，完成胎盘的内分泌、交换、侵袭和着床过程，而基质细胞是胚胎外间充质来源，对绒毛的形成和维持起重要作用。为了研究绒毛膜癌滋养层细胞的功能，建立了不同的细胞系，包括BeWo、JEG-3和JAR，而HTR-8/SVneo是用猴病毒40大T抗原(SV40)感染孕早期绒毛外滋养层细胞建立的。这些细胞系主要用于研究滋养层细胞的功能，包括细胞融合、迁移和侵袭。方法：对HTR-8/SVneo、BeWo、JEG-3和JAR细胞进行免疫荧光、实时定量聚合酶链式反应和免疫印迹分析。结果：HTR-8/SVneo细胞系含有滋养层细胞和基质/间充质细胞。BeWo、JEG-3和Jar细胞均为滋养层/上皮细胞标记CK7阳性，HTR-8/SVneo细胞仅有少量CK7阳性细胞团。有趣的是，在HTR-8/SVneo细胞亚群中检测到波形蛋白的表达，而在所有其他测试的胎盘细胞系中完全不表达波形蛋白。在使用该细胞系研究滋养层细胞功能时，应考虑这种混合的细胞群。(三)2016年。爱思唯尔有限公司版权所有。
Introduction: The placenta, a transient organ in humans, is essential for pregnancy maintenance and fetal development. Trophoblast and stromal cells are the main cell types present in human placenta. Trophoblast cells are derivatives of the trophectoderm layer and fulfill the endocrine, exchange, invasion and implantation processes of the placenta, whereas stromal cells are of extraembryonic mesenchymal origin and are important for villous formation and maintenance. Different cell lines were developed to study trophoblast functions including BeWo, JEG-3 and JAR from chorioncarcinoma while HTR-8/SVneo was developed using first trimester extravillous trophoblast infected with simian virus 40 large T antigen (SV40). These cell lines are largely used to study trophoblast functions including cell fusion, migration and invasion. Therefore, the purity of each cell lines is crucial in order to be able to use them as a model recapitulating trophoblast cells.Methods: HTR-8/SVneo, BeWo, JEG-3 and JAR were analyzed for epithelial and mesenchymal markers using immunofluorescence, real time PCR and Western blot.Results: Our results showed that HTR-8/SVneo cell line contains two populations of cells suggesting the presence of trophoblast and stromal/mesenchymal cells. While all cells in BeWo, JEG-3 and Jar are positive for the trophoblast/epithelial marker CK7, HTR-8/SVneo cells contained few clusters of CK7 positive cells. Interestingly, vimentin expression was detected in a subset of HTR-8/SVneo cells and was completely absent from all other tested placental cell lines.Discussion: Our results unveil the presence of a heterogeneous population of trophoblast and stromal cells within HTR-8/SVneo cell line. This mixed population of cells should be taken into consideration when using this cell line to study trophoblast functions. (C) 2016. Elsevier Ltd. All rights reserved.